hrvatski

Sinteza ružmarinske kiseline u kosmatom korijenju ukrasne koprive Coleus blumei Benth.

Prirodni antioksidans – ružmarinsku kiselinu (RA) nalazimo u vrsta iz porodice Lamiaceae te kod nekoliko vrsta porodice Boraginaceae. Dobro je poznato svojstvo ukrasne koprive da sintetizira značajnu količinu RA. Infekcijom lisnih eksplantata s bakterijom Agrobacterium rhizogenes A4 potaknut je rast kosmatog korijenja. Prisutnost T-DNA u kosmatom korijenju potvrđena je lančanom reakcijom polimeraze (PCR). Tijekom kultivacije u tekućoj hranidbenoj podlozi MS i CB praćeni su rast tkiva i biosinteza RA. Rast transgenog korijenja i biosinteza RA bili su bolji u hranidbenoj podlozi MS. Maksimalni indeks rasta u toj podlozi iznosio je 16, 5 (nakon 42 dana), dok je maksimalni rast korijenja u podlozi CB postignut 49. dan, kada je indeks rasta bio 11, 37. Kosmato korijenje raslo u podlozi MS sintetiziralo je 2, 95 mg RA po tikvici (1, 34% RA u suhoj tvari), u podlozi CB maksimalna sinteza RA po tikvici iznosila je 1, 96 mg RA (1, 15% RA u suhoj tvari). Ovaj rad prvi puta objavljuje sposobnost sinteze RA u kulturi kosmatog korijenja ukrasne koprive. Rosmarinic acid (RA) is a natural antioxidant found especially in plants of Lamiaceae and several Boraginaceae species. Decorative nettle Coleus blumei (Lamiaceae) is well known to produce significant amount of RA. Coleus blumei hairy roots were induced by infection with wild strain Agrobacterium rhizogenes A4. The presence of T-DNA genes in transformed tissue was confirmed by polymerase chain reaction (PCR). The growth of Coleus blumei transformed roots and biosynthesis of RA were analyzed during its cultivation in MS and CB liquid medium. Roots grew faster and better in MS medium and achieved maximum growth index of 16.5, after 42 days. In CB medium maximum growth index of 11.37 was after 49 days. In liquid MS medium hairy roots accumulated maximum 2.95 mg RA per tube, that is about 1.34% RA in dry tissue mass. In CB medium hairy roots accumulated 1.96 mg RA per tube that corresponds to 1.15% RA in dry tissue mass. These is the first report about feasibility of Coleus blumei hairy roots to synthesize RA.

Coleus blumei Benth.

nije evidentirano