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Pregled bibliografske jedinice broj: 186560

Časopis

Autori: Primorac, Dragan; Johnson, Carol V.; Lawrence, Jeanne B.; McKinstry, Monique B.; Stover, Mary Louise; Schanfield, Moses S.; Anđelinović, Šimun; Tadić, Tade; Rowe, David W.
Naslov: Premature termination codon in the aggrecan gene of nanomelia and its influence on mRNA transport and stability
Izvornik: Croatian medical journal (0353-9504) 40 (1999), 4; 528-532
Vrsta rada: članak
Ključne riječi: chondrocytes; codon stop; codon terminator; collagen type II; mRNA; peptide chain termination; RNA; messenger; translation
Sažetak:
Aim. To analyze the influence of the premature termination codon on mRNA transport and stability. Methods. Chondrocyte mRNA was isolated from homozygous and heterozygous nanomelic 17-days old embryos and examined by RT-PCR analysis. To analyze aggrecan mRNA stability, mRNA synthesis was inhibited with DRB [5, 6 dichloro-1-(-D-ribofuranosyl benzimidazole)], a specific inhibitor of RNA polymerase II. Visualization of the aggrecan alleles was performed by in situ hybridization. Results. The level of mutant aggrecan mRNA within the nucleus was equal to that of the control, but no mutant mRNA was observed in the cytoplasm. RT-PCR revealed that the mutant transcript was only detectable in the nucleus, compared with house-keeping glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene or collagen type II. A restriction site induced by premature termination codon TAA allowed the distinction of normal and mutant transcripts in chondrocytes derived from embryos heterozygous for the nanomelic mutation. After the treatment with DRB, identical decay rates were demonstrated for both transcripts within the heterozygous nucleus. In situ hybridization showed no abnormal mRNA accumulation. Conclusion. This is the first evidence suggesting that the transcript of the mRNA with the premature termination codon within an exon does exit the nucleus.
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Puni text rada: 186560.1999_CMJ_Primorac_Johnson_str.528_Premature_termination.pdf (tekst priložen 28. Ožu. 2007. u 14:19 sati)
URL cjelovitog teksta:
Google Scholar: Premature termination codon in the aggrecan gene of nanomelia and its influence on mRNA transport and stability



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