Molecular Recognition Theory of the complementary (antisense) peptide interactions (CROSBI ID 113841)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Štambuk, Nikola ; Konjevoda, Paško ; Boban-Blagaić, Alenka ; Pokrić, Biserka
engleski
Molecular Recognition Theory of the complementary (antisense) peptide interactions
Molecular Recognition Theory is basedon the finding of Blalock et al. (Biochem. Biophys. Res. Commun. 121 (1984) 203-207 ; Nature Med. I (1995)876-878 ; Biochem. J. 234 (1986) 679-683) that peptides specified by the complementary RNAs bind to each other with higher specificity and efficacy. This theory is investigated considering the interaction of the sense peptides coded by means of messenger RNA (read in 5' -3' direction) and antisense peptides coded in 3' -5' direction. We analysed the hydropathy of the complementary amino acid pairs and their frequencies in 1 0 peptide-receptor systems with verified ligand-receptor interaction. An optimization procedure aimed to reduce the number of possible antisense peptides derived from the sense peptide has been proposed. Molecular Recognition Theory was also validated by an "in vivo" experiment. It was shown that 3' -5' peptide antisense of (X-MSH abolished its cytoprotective effects on the gastric mucosa in rats. Molecular Recognition Theory could be useful method to simplify experimental procedures, reduce the costs of the peptide synthesis, and improve peptide structure modelling.
molecular ; recognition ; antisense ; peptide ; RNA
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Podaci o izdanju
123 (4)
2005.
265-275
objavljeno
1431-7613
1611-7530
10.1016/j.thbio.2005.02.001
Povezanost rada
Biologija, Kemija, Temeljne medicinske znanosti