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Pregled bibliografske jedinice broj: 196467

Časopis

Autori: Pavelić, Krešimir; Antonić, Marko; Pavelić, Ljubomir; Pavelić, Jasminka; Pavelić, Zlatko; Spaventi, Šime
Naslov: Human lung cancers growing on extracellular matrix: expression of oncogenes and growth factors
Izvornik: Anticancer research (0250-7005) 12 (1992), 6B; 2191-2196
Vrsta rada: članak
Ključne riječi: human lung cancer ; extracellular matrix
Sažetak:
The goal of this study was to evaluate the etracellular matrix (ECM) as a model for growing human lung cancers and to study the feasibility of its application for cellular and molecular studies of tumor biology. Bovine corneal endothelial cell ECM coated dishes were evaluated as a growth substrate for tumor cultures. Growth success, morphology and oncoprotein/growth factor expression for 74 different lung canscers (adenocarcionoma, epidermoid carcinoma and small cell carcinoma) were compared after seeding fresh surgical explants onto bovine corneal endothelial cell ECM and plastic culture substrate. Ninenteen out of 74 tumors (26%) plated on ECM demonstrated measurable growth. Growth on ECM was superior to growth on plastic for the lung tumors. All 19 tumor cultures showed malignant morphology and functions. They were examined under the light microscope, and in all cases pre-and post-cytology confirmed malignancy. Tumor cells seeded on ECM retained their malignant phenotype in comparison to tumors grown on plastic. Several oncoproteins (c-myc, c-Ha-ras, c-erbB-2) and growth factors/receptors (EGF, EGF-R, TGFalpha) were immunostained. These analyses were performed immediately after disaggregation of tumor cells obtained surgically and after seeding on ECM or plastic. Strong expression of oncoproteins/growth factors was detected in tumor cells immediately after surgery or when the cells were plated on ECM. On the other hand, moderate or no expression was observed in the same type of cells plated on plastic.
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL Internet adrese: http://europepmc.org/abstract/med/1363516
URL cjelovitog teksta:
Google Scholar: Human lung cancers growing on extracellular matrix: expression of oncogenes and growth factors



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