Nested polymerase chain reaction for detection of hepatitis C virus RNA in blood derivatives (CROSBI ID 114039)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Gall-Trošelj, Koraljka ; Kušić, Borka ; Pećina-Šlaus, Nives ; Pavelić, Krešimir ; Pavelić, Jasminka
engleski
Nested polymerase chain reaction for detection of hepatitis C virus RNA in blood derivatives
Testing for the presence of hepatitis C virus (HCV) in blood derivatives used in clinical medicine is of enormous importance to ensure the production of safe preparations. So far, no system has been developed for the isolation and detection of hepatitis C virus from blood derivatives. The recently introduced commercial kit for the detection of hepatitis C virus is designed for the isolation and detection of virus from blood serum. A reliable and reproducible method for the isolation of hepatitis C virus RNA, subsequent reverse transcription and nested polymerase chain reaction (PCR) from blood derivatives is described. Od 17 batches of factor VIII, gama-globulin and anti-tetanus, cytomegalovirus and Varicella-zoster immunoglobulin concentrates, respectively (14 negative for anti HCV and 3 of unknown anti HCV status) five were found positive in RNA-PCR.
nested PCR; hepatitis C virus
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
33 (10)
1995.
733-736-x
objavljeno
0939-4974