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Pregled bibliografske jedinice broj: 196514

Časopis

Autori: Gall-Trošelj, Koraljka; Kušić, Borka; Pećina-Šlaus, Nives; Pavelić, Krešimir; Pavelić, Jasminka
Naslov: Nested polymerase chain reaction for detection of hepatitis C virus RNA in blood derivatives
Izvornik: European journal of clinical chemistry and clinical biochemistry (0939-4974) 33 (1995), 10; 733-736
Vrsta rada: članak
Ključne riječi: nested PCR; hepatitis C virus
Sažetak:
Testing for the presence of hepatitis C virus (HCV) in blood derivatives used in clinical medicine is of enormous importance to ensure the production of safe preparations. So far, no system has been developed for the isolation and detection of hepatitis C virus from blood derivatives. The recently introduced commercial kit for the detection of hepatitis C virus is designed for the isolation and detection of virus from blood serum. A reliable and reproducible method for the isolation of hepatitis C virus RNA, subsequent reverse transcription and nested polymerase chain reaction (PCR) from blood derivatives is described. Od 17 batches of factor VIII, gama-globulin and anti-tetanus, cytomegalovirus and Varicella-zoster immunoglobulin concentrates, respectively (14 negative for anti HCV and 3 of unknown anti HCV status) five were found positive in RNA-PCR.
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL cjelovitog teksta:
Google Scholar: Nested polymerase chain reaction for detection of hepatitis C virus RNA in blood derivatives



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