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 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 197095

Časopis

Autori: Grazio, Simeon; Frković-Grazio, Snježana; Čabrijan, Tomislav; Zjačić-Rotkvić, Vanja; Goldoni, Vesna; Pećina-Šlaus, Nives; Kamenjicki, Elena; Pavelić, Krešimir
Naslov: Freshly frozen subsequently AMeX processed breast carcinoma tissue - a new possibility for immunohistochemical detection of c-myc oncoprotein
Izvornik: Periodicum biologorum (0031-5362) 94 (1992); 215-220
Vrsta rada: članak
Ključne riječi: breast carcinoma tissue; c-myc oncoprotein; immunohistochemical detection
Sažetak:
Normally, oncoprotein p62c-myc is localized in the cell nucleus. In freshly frozen sections, normal immunohistochemical localization of this antigen is preserved but some morphological details have been hampered by destruction pf cell and tissue arhitecture during cryosectioning. To delineate the distribution and intensity of staining for p62c-myc in tumor cells, we used the monoclonal antibody 155-11C7 raised against a synthetic myc peptide. Our results show that the AmeX procedure in previously frozen tissues resulted reproducible nuclear immunostaining for p62c-myc. This finding is important because tissues often kept frozen for steroid receptor analysis can be also used for immunohistochemical localization of oncoproteins.
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
Scopus
SCI-EXP, SSCI i/ili A&HCI
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL cjelovitog teksta:
Google Scholar: Freshly frozen subsequently AMeX processed breast carcinoma tissue - a new possibility for immunohistochemical detection of c-myc oncoprotein



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