Artificial reversion of acute myeloid leukemia cells into normal phenotype (CROSBI ID 114198)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Pavelić, Krešimir ; Baltić, Vladimir ; Spaventi, Šime
engleski
Artificial reversion of acute myeloid leukemia cells into normal phenotype
Induction of tumor cell differentiation could reverse transformed cells into normal, mature cells. Important question is whether these malignant-to-normal reversed cells are really normal ones. We have developed an experimental model based on the examination of three different levels of human acute myeloid leukemia cell properties before and after induction of differentiation: morphological (percentage of undifferentiated blast cells), functional (DNA ploidy, Fc receptors, phagocytic activity, clonogenic assay m soft agar, oxidative metabolism which accompanies phagocytosis in mature granulocytes) and genetical (expression of oncogene p53). Several inducers have been employed: dimethylsulfoxide (DMSO) granulocyte-macrophage colony stimulating factor (GM-CSF) ; tunicamycin, interferon gamma, tumor necrosis factor and lypopolysaccharide. Our results indicate that the reversion of leukemic cells into mature normal ones with some inducers (DMSO, GM-CSF) could be a complete process.
acute myeloid leukemia ; cells
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Podaci o izdanju
22 (5)
1990.
533-538
objavljeno
0020-711X
10.1016/0020-711X(90)90269-9
Povezanost rada
Temeljne medicinske znanosti