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Pregled bibliografske jedinice broj: 197361

Časopis

Autori: Pavelić, Krešimir; Bulbul, M.A.; Slocum, H.K.; Pavelić, Zlatko; Rustum, Y.M.; Niedbala, M.J.; Bernacki, R.J.
Naslov: Growth of human urological tumors on extracellular matrix as a model for the in vitro cultivation of primary human tumor explants
Izvornik: Cancer research (Baltimore) (0008-5472) 46 (1986), 7; 3653-3662
Vrsta rada: članak
Ključne riječi: human urological tumors ; extracellular matrix ; in vitro cultivation of primary human tumor explants
Sažetak:
The goal of this study was to establish an optimal in vitro growth assay system for human urological tumor explants. Bovine corneal endotelial cell extracellular matrix (ECM) coated dishes were evaluated as a growth substrate for tumor cultures. Growth success for different urological carcinomas (prostatic, bladder, kidney, and testicular) was compared after seeding fresh surgical explants onto bovine corneal endothelial cell ECM and plastic culture flasks. Tumor samples were disaggregated enzymatically, and 1 × 104 cells were seeded onto the different substrates using RPMI 1640 medium containing 10% fetal calf serum and/or different growth factors, nutrients, and hormones. Cell growth on ECM was quantitated on days 7–15 by [3H]thymidine uptake, cell counting, and total protein. Tumor cells were characterized by flow cytometry and cytology. It was observed that ECM provides superior culture conditions for urological carcinomas. By increasing the initial number of cells plated on ECM and by adding different growth factors or hormones, the growth rate for specific tumor types was increased significantly. Several tumors (11 cases) grown on ECM were examined under the light microscope, and in all cases pre- and post-cytology confirmed malignancy. Tumor cells maintained on ECM and transplanted into nude mide retained their tumorigenic and morphological characteristics. Clinically aggressive tumors were associated with extensive ECM degradation. In addition, the growth of fresh human tumors on ECM provides a biologically relevant model system (for assessing the invasiveness of tumors in vitro) and should also be useful for drug evaluation studies.
Izvorni jezik: ENG
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bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL Internet adrese: http://cancerres.aacrjournals.org/content/canres/46/7/3653.full.pdf
http://cancerres.aacrjournals.org/content/46/7/3653
URL cjelovitog teksta:
Google Scholar: Growth of human urological tumors on extracellular matrix as a model for the in vitro cultivation of primary human tumor explants



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