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Pregled bibliografske jedinice broj: 212696

Zbornik radova

Autori: Čačev, Tamara; Kapitanović, Sanja; Pavelić, Krešimir.
Naslov: Informativity and results of LOH analysis for five APC gene polymorphic markers.
Izvornik: European Journal of Human Genetics / ICHG (ur.). - Beč : Nature Publishing Group , 2001. 344..
ISSN: 1018-4813
Skup: 10th International Congress of Human Genetics
Mjesto i datum: Beč, Austrija, 15-19.05.2001.
Ključne riječi: APC; LOH; VNTR;
Sažetak:
We examined 46 cases of human sporadic colon cancer and corresponding normal tissue samples to evaluate the LOH at the APC gene loci. DNAs were used for PCR, RFLP, VNTR and LOH analysis. To analyze LOH at the APC gene loci we used five polymorphic markers ; three RFLP intragenic markers (exon 11 RsaI, exon 15 MspI, and exon 15 AspHI) and two VNTR flanking markers (D5S409 and D5S433). The informativity for all three intragenic RFLP markers was 50.0% (23 of 46 assayed), and 21.7% of tumors (5 of 23 informative) demonstrated LOH. The informativity for VNTR flanking markers D5S409 and D5S433 was 60.9% (28 of 46 assayed) and 87.0% (40 of 46 assayed) respectively. Eight of 28 informative tumors (28.6%) demonstrated LOH for marker D5S409, and 14 of 40 informative tumors (35.0%) demonstrated LOH for marker D5S433. The informativity for all five APC loci was 100% and 14 of 46 tumors (30.4%) demonstrated LOH. Our study showed that it was necessary to use VNTR flanking markers D5S409 and D5S433 to increase the number of informative cases (from 50% with intragenic markers) to 100%. The highest informativity was observed for VNTR marker D5S433, 87%.
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SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 0098108
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti



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