Informativity and results of LOH analysis for five APC gene polymorphic markers. (CROSBI ID 510262)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Čačev, Tamara ; Kapitanović, Sanja ; Pavelić, Krešimir.
engleski
Informativity and results of LOH analysis for five APC gene polymorphic markers.
We examined 46 cases of human sporadic colon cancer and corresponding normal tissue samples to evaluate the LOH at the APC gene loci. DNAs were used for PCR, RFLP, VNTR and LOH analysis. To analyze LOH at the APC gene loci we used five polymorphic markers ; three RFLP intragenic markers (exon 11 RsaI, exon 15 MspI, and exon 15 AspHI) and two VNTR flanking markers (D5S409 and D5S433). The informativity for all three intragenic RFLP markers was 50.0% (23 of 46 assayed), and 21.7% of tumors (5 of 23 informative) demonstrated LOH. The informativity for VNTR flanking markers D5S409 and D5S433 was 60.9% (28 of 46 assayed) and 87.0% (40 of 46 assayed) respectively. Eight of 28 informative tumors (28.6%) demonstrated LOH for marker D5S409, and 14 of 40 informative tumors (35.0%) demonstrated LOH for marker D5S433. The informativity for all five APC loci was 100% and 14 of 46 tumors (30.4%) demonstrated LOH. Our study showed that it was necessary to use VNTR flanking markers D5S409 and D5S433 to increase the number of informative cases (from 50% with intragenic markers) to 100%. The highest informativity was observed for VNTR marker D5S433, 87%.
APC; LOH; VNTR;
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
344.-x.
2001.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
European journal of human genetics
ICHG
Beč: Nature publishing group
1018-4813
Podaci o skupu
10th International Congress of Human Genetics
poster
10.05.2001-19.05.2001
Beč, Austrija