engleski

Morphologic determination of apoptosis in cell culture treated with fumonisin B1

Fumonisin B1 is one of several mycotoxins of Fusarium moniliforme fungus which is a major fungal pathogen of corn and widely spread troughout the world. Recent in vivo and in vitro observations have concluded that the toxic effects of FB1 can be investigated trough the appearance of programmed cell death, apoptosis. Cell undergoing apoptosis show characteristic morphological and biochemical features, including ; chromatin aggregation, nuclear and cytoplasmic condensation and partition of cytoplasm and nucleus into membrane bound vesicles ( apoptotic bodies ) which contain ribosomes, morphologically intact mitohondria and nuclear material. The aim of our study was to investigate the time- and dose- dependent ratio of apoptosis, subtoxic doses of FB1 in a short-time cell culture. A cell culture consisting of rabbit kidney cells ( RK-13 ) was used in all of our studies. FB1 was applied in doses of 40nM and 120nM for a duration of 48 to 120 hours. The results indicate that the appearance of apoptosis as a cell reaction to the presence of FB1 in the investigated conditions ( short-time cell culture, low concentration of FB1) is time- and dose-dependent. Despite low concentrations of FB1, a certain effect can be demonstrated on cell viability. On one side, this effect is significant, while, on the other side, it leaves damaged cells enough time and space to program its own "selfdestruction" in the form of apoptosis which will allow the remaining cells to be indifferent and continue their normal life-cycle. This is confirmed by the fact that cells not undrgoing apoptotic changes are morphologically similar to control cells. FB1 in the investigated doses of 40 and 120 nM significantly increased the rate of apoptosis in comparison to control cells in the RK-13 cell culture.

Fumonisins; cell culture; apoptosis

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