A simple and sensitive immunoradiometric assay (IRMA) for detection of foetal calf serum (FCS) proteins in vaccine preparations has been developed. Polyclonal 125-I-labelled anti-FCS was used for detection. The developed assay allows quantification of FCS (down to 10ng/ml) in vaccine preparations (measles vaccine). An enzymeimmunoassay was also developed based on the same assay two-site sandwich principle where anti-FCS was directly labelled with horse-radish peroxidase. ELISA was comparable in sensitivity and accuracy with IRMA |
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