engleski

Prolonged esophagitis in rat: Stable gastric pentadecapeptide BPC 157 (PLD116, PL14736, Pliva) relation to critical period for threatening outcome and failure of lower esophageal and pyloric sphincter

Aim. We focus whether the critical period for threatening outcome in prolonged esophagitis and relation with prolonged failure of lower esophageal sphincter (LES) and pyloric sphincter (PS) could be modified with a stable gastric pentadecapeptide BPC 157 , promising in inflammatory bowel disease (PLD116, PL14736, Pliva). It attenuates esophago-jejunal anastomosis-esophagitis (J Phys, 1999) (1 month), and recovers and maintains esophageal mucosal integrity and sphincters pressure for 1 year (Gastroenterology, 2004) in rats that had grossly intact GI tract with the essential function failure mimicked by tubes into LES and PS. Methods. This procedure induces prominent esophagitis, LES- and PS-malfunction (one tube inserted into LES, and the other tube into PS, for 1 week, then both artlessly removed), gastroduodenal contents reflux. Now, considerably extending esophagitis period, BPC 157 effect was compared in these esophagitis rats with grossly intact GI-tract with essential function failure, after 12 and 20 months, with respect to normal-healthy rats and (i) sphincters pressure (LES, PS) and (ii) esophagitis attenuation. Assessed at 12 or 18 months of esophagitis: (i) LES-, PS- pressure (cm H2O). Water manometer connected with drainage port of Foley catheter was implanted into the stomach either through esophageal or duodenal incision before BPC 157 or saline (1 ml/rat ; 5ml/kg) were given directly into the stomach ; assessment was at 5 min thereafter. (ii) Reflux esophagitis. Macro- (scored 0 (normal) to 4 (the worst)) as well as micro-scopically (program SFORM, VAMSTECH-Software Company, Croatia) ; BPC 157 (10 µ ; g /kg) i.p. once daily or in drinking water. Results. (i) Sphincter pressure. Esophagitis-rats present a marked sphincter dysfunction, constantly lessened LES- and PS-pressure. For both LES and PS, BPC 157 (a) promptly increases the decreased values till the level in healthy, then, (b) maintains pressure preserved at the healthy level during 12-18 months period, while (c) in normal rats, it increases LES- , but decreases PS- pressure (Fig. 1). (ii) Esophagitis-attenuation. BPC 157 attenuates esophageal lesion at either region, and either interval. Thinner epithelium and superficial corneal layer in controls (12-18 months) than in naive healthy and BPC 157 treated animals. Controls (12 months) present a stratification derangement in contrast to healthy controls and BPC treated animals.

BPC 157; esophagitis; sphincter pressure; gastroduodenal reflux

DOI:10.1159/000093383