Biotransformations of Anabolic Steroids Using Recombinant Human CYP3A4 (CROSBI ID 469545)
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Podaci o odgovornosti
Rendić, Slobodan ; Nolteernsting, Eckhardt ; Schänzer Wilhelm
engleski
Biotransformations of Anabolic Steroids Using Recombinant Human CYP3A4
Two formats of human recombinant CYP3A4 have been used for in vitro study of metabolism of anabolic steroids i.e. microsomes prepared from insect cells expressing human enzyme and purified recombinant human CYP3A4 (PANVERA, USA). Structures of metabolites as trimethylsilyl-ether derivatives were determined by the GC/MS method. The reference 6beta-hydroxylated metabolites were synthesized as reported by Schanzer et al., Steroids, 60:353, 1995. After incubation of anabolic steroids with CYP3A4 enzyme systems formation of the following metabolites is suggested: STEROID METABOLITE Testosterone, 6beta-hydroxy- 17alpha-Methyltestosterone 6beta-hydroxy- Methandienone 6beta-hydroxy- 4-Chloro-1,2-dehydro-17alpha-methyltestosterone 6beta-hydroxy- Boldenone monohydroxy- (most probably 6beta-hydroxy-) 5beta-Androstane-3alpha, 17beta-diol 5alpha-dihydrotestosterone (DHT) and monohydroxy- 5beta-Androstane-3alpha, 17beta-iol no metabolites identified 5alpha-Dihydrotestosterone (DHT) monohydroxy- Androst-4-ene-3,17-dion mono- and bishydroxy- Androst-4-ene-3beta,17beta-diol testosterone and monohydroxy- Androst-5-ene-3beta,17beta-diol no metabolites identified The results indicate that 6beta-hydroxylation is a common and preferential metabolic pathway for 3-one-, 4-ene-steroids when incubated with human CYP3A4 enzyme in vitro.
Biotransformations of Anabolic Steroids Using Recombinant Human CYP3A4
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Podaci o prilogu
81-81-x.
1999.
objavljeno
Podaci o matičnoj publikaciji
Bethesda (MD): International Society for the Study of Xenobiotics
Podaci o skupu
Nepoznat skup
poster
29.02.1904-29.02.2096