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Pregled bibliografske jedinice broj: 256612

Časopis

Autori: Boens, Noël; Qin, Wenwu; Basarić, Nikola; Orte, Angel; Talavera, Eva M.; Alvarez-Pez, Jose M.
Naslov: Photophysics of the Fluorescent pH Indicator BCECF
Izvornik: The Journal of Physical Chemistry A (1089-5639) 110 (2006), 30; 9334-9343
Vrsta rada: članak
Ključne riječi: absorption; BCECF; fluorescence; global analysis; photophysics; pH
Sažetak:
The photophysical behavior of BCECF [2', 7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein] − currently the most widely used fluorescent pH indicator for near-neutral intracellular pH measurements – has been explored by using absorption and steady-state and time-resolved fluorescence measurements. The influence of ionic strength as well as total buffer concentration on the absorbance and steady-state fluorescence has been investigated. The apparent acidity constant of the pH indicator determined by absorbance and fluorescence titration is dependent on the added buffer and salt concentrations. A semi-empirical model is proposed to rationalize the variations in the apparent pKa values. The excited-state proton exchange of BCECF at physiological pH becomes reversible upon addition of phosphate buffer inducing a pH dependent change of the fluorescence decay times. Fluorescence decay traces collected as a function of total buffer concentration and pH were analyzed by global compartmental analysis yielding the following values of the rate constants describing excited-state dynamics of BCECF: k01 = 3.4 x 108 s− 1, k02 = 2.6 x 108 s− 1, k21 = 1 x 106 M− 1 s− 1, = 1.4 x 108 M− 1 s− 1, and = 4.3 x 107 M− 1 s− 1.
Projekt / tema: 0098052
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kemija
URL cjelovitog teksta:
Google Scholar: Photophysics of the Fluorescent pH Indicator BCECF



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