engleski

Prevalence of periodontal pathogens in Croatian generalized chronic and aggressive periodontitis

Aim: To investigate the prevalence of A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythensis and T. denticola in generalized chronic and aggressive periodontitis patients. Materials and methods: Seventy-four patients were divided in two groups: (i) generalized chronic periodontitis (CP, n = 39) and (ii) generalized aggressive periodontitis (GAgP, n = 34). One examiner (D.B.) using standard periodontal probe recorded at four sites per tooth bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL) values. Four subgingival plaque samples were collected from the deepest pockets in each quadrant. Microbiological testing was done utilizing the multiplex PCR of 16S rDNA followed by a simultaneous reverse hybridization to identify five periodontal pathogens in a single run. Results: The average age for CP was 48.18 and for GAgP 32.97. At sampling sites PPD for the CP group was 7.73 mm, CAL was 8.49, for the GAgP group 7.74 mm and 8.36 mm, respectively. Our results show that the isolation frequency of pathogens in the CP group A.a 23%, P.g. 87.17%, P.i. 51.28%, T.f. 92.3% and T.d.71.79%, and for the GAgP A.a 40%, P.g. 71.42%, P.i. 48.57%, T.f. 85.71% and T.d. 77.14%. Conclusions: We could not find any differences between the groups except for A.a. (40% in GAgP vs. 23% in CP). It seems that the main pathogens in our population for the CP and GAgP groups are the red complex bacteria with T.f. being the most frequent.

periodontitis; periodontal pathogens

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