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 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 268703

Časopis

Autori: Cvek, Josipa; Medić-Šarić, Marica; Jasprica, Ivona; Zubčić, Snježana; Vitali, Dubravka; Mornar, Ana; Vedrina-Dragojević, Irena; Tomić, Siniša
Naslov: Optimization of extraction procedure and chemical characterization of Croatian propolis tinctures
( Optimization of extraction procedure and chemical characterization of Croatian propolis tinctures )
Izvornik: Phytochemical Analysis (0958-0344) 18 (2007), 5; 451-459
Vrsta rada: članak
Ključne riječi: Polyphenols; spectrophotometric methods; validation; quality control
( Polyphenols; spectrophotometric methods; validation; quality control )
Sažetak:
Three spectrophotometric methods for the quantitative determination of different flavonoid groups and total phenolics in Croatian propolis samples were optimized and validated. The assay based on the formation of aluminium chloride complex (with galangin as a standard) was applied for the quantification of flavones and flavonols, while 2, 4-dinitrophenylhidrazine method (with pinocembrine as a reference) was used for the quantification of flavanones. Total phenolics content was measured by the Folin-Ciocalteau method using reference solution of caffeic acid : galangin : pinocembrine (1:1:1). Through analytical validation, the most suitable extraction conditions (regarding the time, temperature, and concentration of extraction solvent) were determined, and final conditions for the extraction were established (80% ethanol, 1 hour at the room temperature). Appropriate ratio between the mass of raw propolis and extraction solvent volume was established as well. Applying developed method of extraction, ten propolis tinctures were prepared and subjected to the analysis of general pharmacopoeial parameters which are fundamental for the creation of quality specification (relative density, dry residue of extract, content of ethanol, methanol and 2-propanol). Additionally, we determined the content of waxes as the main inactive constituents in order to observe the level of their migration from crude propolis to the prepared tinctures.
Projekt / tema: 0006541
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Farmacija
URL cjelovitog teksta:
Google Scholar: Optimization of extraction procedure and chemical characterization of Croatian propolis tinctures



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