Immunohistochemistry of hepatic gangliosides following a partial hepatectomy and exposure to different hyperbaric oxygen treatments (CROSBI ID 522741)
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Podaci o odgovornosti
Tičinović Kurir, Tina ; Čikeš Čulić, Vedrana ; Zemunik, Tatijana ; Grković, Ivica ; Petri, Nadan ; Markotić, Anita
engleski
Immunohistochemistry of hepatic gangliosides following a partial hepatectomy and exposure to different hyperbaric oxygen treatments
It has been shown that gangliosides play an important functional role in the liver growth regulation. In this study we performed immunohistochemical analysis of liver gangliosides on weanling rats following a 15% partial hepatectomy and different pre- and post-operative hyperbaric oxygenation treatments. Frozen sections of liver tissues were analysed with confocal microscopy after staining with five antibodies, specific for gangliosides GM3(Neu5Ac), GM3(Neu5Gc), nLc4Cer, GM2(Neu5Ac), and anti-GalNAc-GM1b, with or without permeabilization. All antibodies were capable of labelling of regenerated hepatocytes, predominantly at the cell surface level. The strongest reactivity was observed for GM3(Neu5Gc), equally in all groups and in all animals. Following a permeabilization with organic solvent the anti-GM3(Neu5Ac) antibody displayed different labeling pattern in pre-operative oxygenated animals ; the staining was detected in the perinuclear area of hepatocytes. The same group showed the highest expression of nLc4 glycoantigen. GM2 and GalNAc-GM1b gangliosides were clearly detected in permeabilized hepatocytes of pre-operative oxygenated animals while same glycoantigens were weakly expressed in all other groups. Alternation in the expression of gangliosides following a partial hepatectomy suggests that gangliosides play an important role in liver growth regulation and may be utilised as markers for the liver recovery after partial hepatectomy.
hyperbaric oxygenation; liver regeneration; gangliosides
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Podaci o prilogu
50-50.
2006.
objavljeno
Podaci o matičnoj publikaciji
EMBO/HHMI Central European Scientists Meeting 2006. : abstract book
Zagreb:
Podaci o skupu
EMBO/HHMI Central European Scientists Meeting
poster
15.06.2006-17.06.2006
Cavtat, Hrvatska