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Pregled bibliografske jedinice broj: 291525

Časopis

Autori: Boens, Noël; Qin, Wenwu; Basarić, Nikola; Hofkens, Johan; Ameloot, Marcel; Pouget, Jacques; Lefevre, Jean-Pierre; Valeur, Bernard; Gratton, Enrico; VandeVen, Martin; Silva, Jr., Norberto D.; Engelborghs, Yves; Willaert, Katrien; Sillen, Alain; Rumbles, Garry; Phillips, David; Visser, Antonie J. W. G.; van Hoek, Arie; Lakowicz, Joseph R.; Malak, Henryk; Gryczynski, Ignacy; Szabo, Arthur G.; Krajcarski, Don T.; Tamai, Naoto; Miura, Atsushi
Naslov: Fluorescence Lifetime Standards for Time and Frequency Domain Fluorescence Spectroscopy
Izvornik: Analytical chemistry (0003-2700) 79 (2007), 5; 2137-2149
Vrsta rada: članak
Ključne riječi: fluorescence lifetime standards; phase-modulation fluorometry; single photon timing
Sažetak:
A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multi-frequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, and water). These lifetime standards are anthracene, 9-cyanoanthracene, 9, 10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide (NATA), 1, 4-bis(5-phenyloxazol-2-yl)benzene (POPOP), 2, 5-diphenyloxazole (PPO), rhodamine B, rubrene, N-(3-sulfopropyl)acridinium (SPA), and 1, 4-diphenylbenzene (p-terphenyl). At 20 °C the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 nm to 575 nm, the emission from 330 nm to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for twenty {; ; ; ; fluorescence lifetime standard/solvent}; ; ; ; combinations are given.
Projekt / tema: 098-0982933-2911
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kemija
URL cjelovitog teksta:
Google Scholar: Fluorescence Lifetime Standards for Time and Frequency Domain Fluorescence Spectroscopy



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