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Pregled bibliografske jedinice broj: 327560

Časopis

Autori: Šegvić Klarić, Maja; Rumora, Lada; Ljubanović, Danica; Pepeljnjak, Stjepan
Naslov: Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment
( Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment )
Izvornik: Archives of Toxicology (0171-9750) 82 (2008), 4; 247-255
Vrsta rada: članak
Ključne riječi: Fumonisin B1; Beauvericin; Ochratoxin A; Necrosis; Apoptosis; Caspase-3; Mycotoxin interactions
( Fumonisin B1; Beauvericin; Ochratoxin A; Necrosis; Apoptosis; Caspase-3; Mycotoxin interactions )
Sažetak:
The objective of this study was to determine individual and combined effects of fumonisin B(1) (FB(1)), beauvericin (BEA) and ochratoxin A (OTA) on porcine kidney epithelial PK15 cell survival by measuring lactate dehydrogenase (LDH) activity, apoptotic index and caspase-3 activity. Cells were treated with 0.05, 0.5 and 5 mug/ml of each mycotoxin or with the combinations of two or all three mycotoxins for 24 and 48 h. Changes in LDH and caspase-3 activity, and in apoptotic index showed that the cytotoxic and apoptotic effects of these mycotoxins were concentration- and time- dependent. Significant increase of LDH activity was observed after 48 h of exposure to the highest concentration of FB(1) (45%), BEA (84%) and OTA (77%), as compared to control. OTA increased caspase-3 activity after 24 h of treatment with 0.5 mug/mL (84%), while BEA (319%) and FB(1) (419%) significantly affected this enzyme activity after 48 h (P < 0.05). Increase of caspase-3 activity preceded significant morphological apoptotic changes, which were detected after 48 h of exposure to a single toxin. Combined treatment with FB(1), BEA and OTA resulted mostly in additive effects on LDH activity, and additive and synergistic effects on caspase-3 activity and apoptotic index.
Projekt / tema: 006-0061245-0977, 006-0061117-1242
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Farmacija
URL Internet adrese: http://www.springerlink.com/content/l6836612n755v26x/fulltext.pdf
URL cjelovitog teksta:
Google Scholar: Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment
Upisao u CROSBI: Lada Rumora (lrumora@pharma.hr), 3. Tra. 2008. u 13:33 sati



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