crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 337495

Časopis

Autori: Petrović, Vlatka; Perez-Garcia, Concepcion; Pasantes, Juan; Šatović, Eva; Prats, Eva; Plohl, Miroslav
Naslov: A GC-rich satellite DNA and karyology of the bivalve mollusc Donax trunculus: a dominance of GC-rich heterochromatin
Izvornik: Cytogenetics and Genome Research (1424-8581) 124 (2009), 1; 63-71
Vrsta rada: članak
Ključne riječi: tandem repeats; subtelomeric heterochromatin; fluorochrome banding; FISH
Sažetak:
We characterized the DTF2 satellite DNA family of the clam Donax trunculus and compared its chromosomal localization with cytogenetic data revealed by fluorochrome banding, C-banding and 28 S rDNA FISH. In contrast to the other satellites detected previously in this species, DTF2 is an abundant (2 %) GC rich satellite that exhibits CpG methylation. Sequence characteristics of DTF2 indicate that its evolution is not affected by constraints that might indicate some functional interactions. Fluorescence in situ hybridization revealed subtelomeric location of this satellite on a subset of 14 out of 19 D. trunculus chromosome pairs. The chromomycin A3 (CMA) staining of GC-rich regions on D. trunculus chromosomes revealed a complex banding pattern that overlaps completely with C-bands. In total, only three bands show subtelomeric location, while 13 bands are located interstitially, one of them being coincident with the 28S rDNA hybridization signal. No bands, either CMA positive (GC-rich) or DAPI positive (AT-rich) were detected at centromeric chromosomal positions. Only two of the CMA-positive bands co-localize with DTF2 satellite, showing (i) the presence of small islands of GC-rich repetitive sequences that remained undetected by CMA/C-banding, and (ii) the abundance of DTF2-divergent GC-rich sequences at interstitial chromosomal locations.
Projekt / tema: 098-0982913-2756
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Biologija
URL Internet adrese: http://www.online.karger.com/ProdukteDB/produkte.asp?Aktion=Ausgabe&Ausgabe=245135&ProduktNr=224037
Broj citata:
Altmetric:
DOI: 10.1159/000200089
URL cjelovitog teksta:
Google Scholar: A GC-rich satellite DNA and karyology of the bivalve mollusc Donax trunculus: a dominance of GC-rich heterochromatin
Upisao u CROSBI: plohl@irb.hr (plohl@irb.hr), 5. Svi. 2008. u 13:48 sati



  Verzija za printanje   za tiskati


upomoc
foot_4