crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 346939

Časopis

Autori: Čipak, Ana; Jaganjac, Morana; Tehlivets, Oksana; Kohlwein, Sepp D.; Žarković, Neven
Naslov: Adaptation to oxidative stress induced by polyunsaturated fatty acids in yeast
Izvornik: Biochimica et Biophysica Acta-Molecular & Cell Biology of Lipids (1388-1981) 1781 (2008), 6/7; 283-287
Vrsta rada: članak
Ključne riječi: fatty acid peroxidation; Reactive oxygen species; Saccharomyces cerevisiae; fatty acid desaturase; catalase
Sažetak:
To create a conditional system for molecular analysis of effects of polyunsaturated fatty acids (PUFA) on cellular physiology, we have constructed a strain of yeast (Saccharomyces cerevisiae) that functionally expresses, under defi ned conditions, the Δ 12 desaturase gene from the tropical rubber tree, Hevea brasiliensis. This strain produces up to 15% PUFA, exclusively under inducing conditions resulting in production of 4-hydroxy-2-nonenal, one of the major end products of n− 6 polyunsaturated fatty acid peroxidation. The PUFA-producing yeast was initially more sensitive to oxidative stress than the wild-type strain. However, over extended time of cultivation it became more resistant to hydrogen peroxide indicating adaptation to endogenous oxidative stress caused by the presence of PUFA. Indeed, PUFA-producing strain showed an increased concentration of endogenous ROS, while initially increased hydrogen peroxide sensitivity was followed by an increase in catalase activity and adaptation to oxidative stress. The deletion mutants constructed to be defective in the catalase activity lost the ability to adapt to oxidative stress. These data demonstrate that the cellular synthesis of PUFA induces endogenous oxidative stress which is overcome by cellular adaptation based on the catalase activity
Projekt / tema: 098-0982464-2519, 108-0000000-0028
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL Internet adrese: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VNN-4S80XPB-2-9&_cdi=6183&_user=3875467&_orig=search&_coverDate=07%2F31%2F2008&_sk=982189993&view=c&wchp=dGLzVlz-zSkzk&md5=7cc62863ecb20c577b4ea832f3b97ab7&ie=/sdarticle.pdf
Broj citata:
Altmetric:
DOI: 10.1016/j.bbalip.2008.03.010
URL cjelovitog teksta:
Časopis izlazi u samo elektroničkom izdanju: NE
Google Scholar: Adaptation to oxidative stress induced by polyunsaturated fatty acids in yeast
Upisao u CROSBI: mstroser@mef.hr (mstroser@mef.hr), 9. Svi. 2008. u 12:55 sati



  Verzija za printanje   za tiskati


upomoc
foot_4