engleski

Population study at STR loci Penta D and Penta E in Croatian population

In addition to our previous population studies of Croatian human population at 15 STR loci included in the AmpFlSTR® ; Identifiler® ; system we have also analyzed the allele frequency distribution at two additional STR loci Penta D and Penta E in the representative sample of Croatian population since these highly polymorphic pentanucleotide loci included in the PowerPlex® ; 16 enhance the discrimination power of that system, which is important in resolving paternity disputes, and are also ideal loci for evaluation of DNA mixtures often encountered in forensic casework. A total of 200 unrelated Caucasian individuals born in Croatia have been sampled for the analysis. Buccal swabs have been used as the DNA source. The QuiAmp DNA blood mini kit was used for DNA extraction. The PowerPlex® ; 16 System has been used to simultaneously amplify 15 STR loci: Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Penta D, CSF1PO, D16S539, D7S820, D13S317, D5S818 and Amelogenin. STR loci were amplified in ABI GeneAmp® ; PCR Thermal Cycler according to the manufacturer’ s recommendations. Electrophoresis of the amplified products was preformed on an ABI 3130 genetic analyzer. Raw data have been compiled, analyzed and numerical allele designations of the profiles were obtained by using the accessory software: ABI 3130 Genetic Analyzer Data Collection v3.0 and GeneMapper  ID Software v3.1. Deviation from Hardy-Weinberg equilibrium, observed and expected heterozygosity, power of discrimination and power of exclusion were calculated. We have also compared our data with data obtained from geographically neighboring European populations.

DNA typing; STR; Croatian population data; PowerPlex 16

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