engleski

Comparison of different polymerase chain reaction methods for detection of human papillomaviruses

Purpose: Many epidemiologic studies and basic research on molecular level strongly support the role of human papillomaviruses as etiologic agents in cervical carcinogenesis. There are more than 70 HPV types of which more than 30 infect genital sites and have different oncogenic potential. Diagnosis of HPV infections is of extreme importance in the prevention of cervical cancer. Methods: We tested the presence of HPV DNA by polymerase chain reaction (PCR) in cervical scrapes obtained from consenting women with cytomorphologically abnormal cervical smears. In order to evaluate different PCR approaches for screening and detection of HPVs we used and then compared the results obtained with three sets of general primers localised within the L1 region of HPV genome (MY09/MY11, inosine-containing MY09/MY11 and L1C1/L1C2) on 164 samples. These results have also been compared with results obtained with type-specific primer pairs for HPV types 6, 11, 16, 18, 31 and 33. Results: HPV DNA was detected in 125/164 (76.22%) cervical scrapes (positive result with at least one set of consensus primers) ; the concordance of results obtained with three sets of general primers (either positive or negative result) was 53.05%. HPV type was determined in 97/164 (59.15%) samples ; in 16/164 (10.37%) samples a multiple HPV infection was found. Conclusion: Simultaneous use of MY09/MY11 and L1C1/L1C2 primer sets in combination with type specific PCR is a valuable method for HPV screening and typing.

HPV; PCR

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