crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 397510

Časopis

Autori: Sanson, Marie; Augé, Nathalie; Vindis, Cécile; Muller, Carole; Bando, Yoshio; Thiers, Jean-Claude; Marachet, Marie-Agnè s; Žarković, Kamelija; Sawa, Yoshiki; Salvayre, Robert; Nè gre-Salvayre, Anne
Naslov: Oxidized Low-Density Lipoproteins Trigger Endoplasmic Reticulum Stress in Vascular Cells : Prevention by Oxygen-Regulated Protein 150 Expression
Izvornik: Circulation research (0009-7330) 104 (2009), 3; 328-336
Vrsta rada: članak
Ključne riječi: ER stress; apoptosis; ORP150; oxidized LDL; atherosclerosis
Sažetak:
Oxidized low-density lipoproteins (oxLDLs) trigger various biological responses potentially involved in atherogenesis. Disturbing endoplasmic reticulum (ER) function results in ER stress and unfolded protein response, which tends to restore ER homeostasis but switches to apoptosis when ER stress is prolonged. We aimed to investigate whether ER stress is induced by oxLDLs and can be prevented by the ER-associated chaperone ORP150 (150-kDa oxygen-regulated protein). oxLDLs and the lipid oxidation products 7-ketocholesterol and 4-hydroxynonenal induce ER stress in human endothelial cells (HMEC-1), characterized by the activation of ER stress sensors (phosphorylation of Ire1 and PERK, nuclear translocation of ATF6) and of their subsequent pathways (eukaryotic initiation factor 2 phosphorylation, expression of XBP1/spliced XBP1, CHOP, and KDEL chaperones GRP78, GRP94, ORP150). ER stress was inhibited by the antioxidant N-acetylcysteine. In advanced atherosclerotic lesions, phospho-Ire1 , KDEL, and ORP150 staining were localized in lipid-rich areas with 4-hydroxynonenal adducts and CD68-positive macrophagic cells. By comparison, staining for 4-hydroxynonenal, phospho-Ire1 , KDEL, and ORP were faint and more diffuse in intimal hyperplasia. ER stress takes part in the apoptotic effect of oxLDLs, through the Ire1 /c-Jun N-terminal kinase pathway, as assessed by the protective effect of specific small interfering RNAs and c-Jun N-terminal kinase inhibitor. Forced expression of the chaperone ORP150 reduced both oxLDL-induced ER stress and apoptosis. ER stress markers and ORP150 chaperone are expressed in areas containing oxLDLs in atherosclerotic lesions and are induced by oxLDLs and oxidized lipids in cultured cells. The forced expression of ORP150 highlights its new protective role against oxLDL-induced ER stress and subsequent apoptosis.
Projekt / tema: 098-0982464-2519, 108-0000000-0028
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Broj citata:
Altmetric:
DOI: 10.1161/CIRCRESAHA.108.183749
URL cjelovitog teksta:
Google Scholar: Oxidized Low-Density Lipoproteins Trigger Endoplasmic Reticulum Stress in Vascular Cells : Prevention by Oxygen-Regulated Protein 150 Expression
Upisao u CROSBI: mstroser@mef.hr (mstroser@mef.hr), 1. Lip. 2009. u 14:49 sati



  Verzija za printanje   za tiskati


upomoc
foot_4