Role of AQP1 in endotoxemia-induced acute kidney injury (CROSBI ID 153665)
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Podaci o odgovornosti
Wang, W. ; Li, C. ; Summer, S.N. ; Falk, S. ; Wang, W. ; Ljubanović, Danica ; Schrier, R.W.
engleski
Role of AQP1 in endotoxemia-induced acute kidney injury
The effect of endotoxemia (lipopolysaccharide, 2.5 mg/kg ip) was investigated in aquaporin (AQP) 1 knockout (KO) compared with wild-type (WT) mice. At baseline, KO mice exhibited higher water intake (WI) and urine output (UO). After endotoxemia, WI and UO remained higher in the KO than WT mice, and urine osmolality was lower. The higher serum osmolality in AQP1-KO mice during endotoxemia was associated with higher AQP2 (133 ± ; 8 vs. 100 ± ; 3%, P < 0.01), AQP3 (140 ± ; 8 vs. 100 ± ; 4%, P < 0.001) and Na+-K+-2Cl– cotransporter type 2 (NKCC2 ; 152 ± ; 14 vs. 100 ± ; 15%, P < 0.05) expression than that in WT mice. These responses during endotoxemia in the AQP1-KO mice compared with WT were associated with lower glomerular filtration rate (GFR) (69 ± ; 8 vs. 96 ± ; 8 ml/min, P < 0.05) and renal blood flow (0.77 ± ; 0.1 vs. 1.01 ± ; 0.1 ml/min, P < 0.01). Urinary sodium excretion and fractional sodium excretion were higher in KO compared with WT mice in endotoxemia and were accompanied by more severe tubular injury. With water repletion and comparable serum osmolalities, GFR was still lower in KO (57 ± ; 13 vs. 120 ± ; 6 ml/min, P < 0.01) compared with WT during endotoxemia. The abundance of AQP2 and AQP3 protein in KO mice was not different from WT mice ; however, NKCC2, Na+/H+ exchanger type 3, and fractional sodium excretion remained higher in KO compared with WT. Thus the polyuria in AQP1-KO mice does not protect against endotoxemia-induced acute kidney injury but rather absence of AQP1 predisposed to enhanced endotoximic renal injury.
lipopolysaccharide; sepsis; glomerular filtration rate; polyuria; aquaporin-1
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Podaci o izdanju
294 (6)
2008.
F1473-14801
objavljeno
1931-857X
10.1152/ajprenal.00036.2008
Povezanost rada
Temeljne medicinske znanosti