Trametes versicolor is white-rot fungus belonging to wood-decomposing basidiomycetes. It has a powerful extracellular enzymatic complex mainly comprised of lignin-peroxidase, manganase- peroxidase and laccase [1]. Laccases (EC 1.10.3.2, p-diphenol: dioxygen oxidoreductases) are multi- copper proteins that catalyze oxidation of wide range of substrates by a radical-catalyzed reaction mechanism They are considered as environmentally friendly enzymes while they require air, produce water as it’ s only by products, possess relatively high reaction rates and show broad substrate specificity. Therefore laccases have gained a lot of attention in many industrial and environmental fields such as food industry, pulp and paper industry, textile, nanotechnology, soil bioremediation, synthetic chemistry, cosmetics, ethanol production, wine clarification, alkenes oxidation, herbicide degradation, diagnostic assays, herbal medicine etc. [2, 3]. Since these applications require of large amounts of enzymes at low cost, one of the possible approaches for decreasing the costs of productions is the utilization of cheap and easily available substrates such as lignocellulosic wastes or by products [4]. In this work, waste from pulp and paper industry has been used in Trametes versicolor MZKI G-99 cultivation with the aim of laccase production. Experiments were carried out in a batch mode on rotary shaker in Erlenmeyer flasks. A high laccase activity (2378 U dm-3) was achieved.

Biotehnologija