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Pregled bibliografske jedinice broj: 430975

Časopis

Autori: Pleše, Bruna; Schröder, Heinz C.; Grebenjuk, Vladislav A.; Wegener, Gerhard; Brandt, David; Natalio, Filipe; Müller, Werner E. G.
Naslov: Strombine dehydrogenase in the demosponge Suberites domuncula: Characterization and kinetic properties of the enzyme crucial for anaerobic metabolism
Izvornik: Comparative Biochemistry and Physiology Part B, Biochemistry and Molecular Biology (1096-4959) 154 (2009), 1; 102-107
Vrsta rada: članak
Ključne riječi: Anaerobic metabolism; Demospongiae; Opine dehydrogenase; Strombine dehydrogenase; Suberites domuncula
Sažetak:
Previously, the cDNA and the respective gene for a presumed tauropine dehydrogenase (TaDH) from Suberites domuncula (GenBank accession nos. AM712888, AM712889) had been annotated. The conclusion that the sequences encode a TaDH had been inferred from the 68% identity with the TaDH protein from the marine demosponge Halichondria japonica. However, subsequent enzymatic assays shown here indicate that the presumed S. domuncula opine dehydrogenase is in fact a strombine dehydrogenase (StDH). The enzyme StDH is highly specific for glycine and is inhibited by an excess of the substrate pyruvate. Besides kinetic data, we report in this study also on the predicted tertiary and quaternary structure of the sponge StDH. It is concluded that the dimer (75 kDa) has a novel structure, distinguishing it from other known marine invertebrate OpDHs that exist as monomers.
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Biologija
URL Internet adrese: http://www.ncbi.nlm.nih.gov/pubmed/19454319
Broj citata:
Altmetric:
DOI: 10.1016/j.cbpb.2009.05.008
URL cjelovitog teksta:
Google Scholar: Strombine dehydrogenase in the demosponge Suberites domuncula: Characterization and kinetic properties of the enzyme crucial for anaerobic metabolism
Upisao u CROSBI: bplese@irb.hr (bplese@irb.hr), 20. Lis. 2009. u 11:38 sati



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