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Pregled bibliografske jedinice broj: 430994

Zbornik radova

Autori: Pleše, Bruna; Grebenjuk, Vladislav A.; Brandt, David; Müller, Werner E. G.
Naslov: Evolution of anaerobic pathways/Opine pathways in sponge S. domucula.
Izvornik:
Skup: Summer school of Marie Curie Training Network (MCTN) “ Biocapital”
Mjesto i datum: Rovinj, Hrvatska, 19-26.08.2007.
Ključne riječi: Anaerobic pathways; Porifera; opine dehydrogenase; Strombine dehydrogenase;
Sažetak:
StDH (strombine dehydrogenase) is an opine dehydrogenase which is involved in opine production pathways found mainly in marine invertebrates. These anaerobic pathways are regarded as analogues to the classical anaerobic glycolytic pathway (lactate production pathway), which is predominant in vertebrates. StDH gene is described for the first time in Eukaryotes. Moreover, two allelic variants have been identified which are present in the different specimens either as a homozygotic or a heterozygotic. Phylogenetic analyses supported with enzymatic assays indicate that S. domuncula StDH is only distantly related to the opine dehydrogenases from marine invertebrates. StDH showed that the protein is highly specific to glycine and inhibited by the substrate pyruvate. Furthermore, S. domunucla StDH has a dimeric structure (75 kDa) which is not observed in so far described OpDHs that are monomeric proteins. This enzyme showed similarities to the OCD/mu-cristallyin protein family. Results showed that a sponge StDH is unusual enzyme that belongs to the independent enzyme class. In addition, expression studies revealed that the StDH is down-regulated with aeration. Immunohistology analyses showed high expression of the protein in almost all sponge cells. A strong accumulation of the enzyme was seen around the bacteria indicating that under aerobic conditions the bacteria might metabolize strombine (end product of the reaction).
Vrsta sudjelovanja: Pozvano
Vrsta prezentacije u zborniku: Nije objavljen
Vrsta recenzije: Međunarodna recenzija
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Kemija,Biologija
Upisao u CROSBI: bplese@irb.hr (bplese@irb.hr), 20. Lis. 2009. u 13:12 sati



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