The main goal of this research was to establish the influence of 8 % (PN-8) and 16 % (PN-16) rape seed oil-cake in feed mixture on production of hens and fatty acids content of eggs yolk. 90 hens of Hrvatica strain have been used in period of 24th to 32th weeks of egg production. Hens have been sorted out in 30 cages, 3 of them in each cage. Hens from group PN-16 laid significantly (P<0.05) lighter eggs and had highest consumption of feed mixture/kg of eggs. The nutrition treatment had not clearly negative effect on average egg production during the investigation. The mortality of 10% in group PN-16 and 6.67 % in group PN-8, points to the possible negative impact of rape seed oil cakes on the health of hens. Feeding hens the diet with rapeseed oil-cake decreased (P<0.05) SFA, increased PUFA n-3 and PUFA n-6 content of egg yolks and had positive effect (P<0.05) on PUFA n-6/PUFA n-3 ratio. Taking into consideration above mentioned, it can be concluded that the rape seed oil-cake can be recommended to be used in the portion of 8 % in feed mixture for Hrvatica laying hens. (Keywords: rape seed oil-cake, laying hens, production, fatty acid, eggs)

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