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Pregled bibliografske jedinice broj: 488010

Časopis

Autori: Breusing, Nicolle; Grune, Tilman; Andrišić, Luka; Atalay, Mustafa; Bartosz, Grzegorz; Biasi, Fiorella; Borović, Suzana; Bravo, Laura; Casals, Isidre; Casillas, Rosario; Dinischiotu, Anca; Drzewinska, Joanna; Faber, Heidemarie; Mohd Fauzi, Norsyahida; Gajewska, Agnieszka; Gambini, Juan; Gradinaru, Daniela; Kokkola, Tarja; Lojek, Antonin; Luczaj, Wojciech; Margina, Denisa; Mascia, Cinzia; Mateos, Raquel; Meinitzer, Andreas; Mitjavila, María Teresa; Mrakovčić, Lidija; Munteanu, Maria Cristina; Podborska, Martina; Poli, Giuseppe; Sicinska, Paulina; Skrzydlewska, Elzbieta; Vina, Jose; Wiswedel, Ingrid; Žarković, Neven; Zelzer, Sieglinde; Spickett, Corinne M.
Naslov: An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples
Izvornik: Free radical research (1071-5762) 44 (2010), 10; 1203-1215
Vrsta rada: pregledni rad
Ključne riječi: oxidative stress; F2-Isoprostanes; 4.-hydroxynonenal; malondialdehyde
Sažetak:
Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F 2 -isoprostanes are widely used as markers of oxidative stress in vitro and in vivo . This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coeffi cients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.
Projekt / tema: 098-0982464-2519
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kemija,Biologija,Temeljne medicinske znanosti
URL Internet adrese: http://informahealthcare.com/doi/abs/10.3109/10715762.2010.499907
Broj citata:
Altmetric:
DOI: 10.3109/10715762.2010.499907
URL cjelovitog teksta:
Google Scholar: An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples
Upisao u CROSBI: acipak@irb.hr (acipak@irb.hr), 25. Lis. 2010. u 13:54 sati



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