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Pregled bibliografske jedinice broj: 503707

Zbornik radova

Autori: Pantović, Radojka; Tota, Marin; Sigler, Melani; Milin, Čedomila
Naslov: Effect of olive oil enriched diet on fatty acid composition of brain phospholipids from mice during liver regeneration
( Effect of olive oil enriched diet on fatty acid composition of brain phospholipids from mice during liver regeneration )
Izvornik: XXII. HRVATSKI SKUP KEMIČARA I KEMIJSKIH INŽENJERA / XXII CROATIAN MEETING OF CHEMISTS AND CHEMICAL ENGINEERS : Knjiga sažetaka / Book of Abstracts / Zagreb 2011
Skup: XXII. HRVATSKI SKUP KEMIČARA I KEMIJSKIH INŽENJERA
Mjesto i datum: Zagreb, Hrvatska, 13.-16. 02. 2011.
Ključne riječi: diet; phospholipids; fatty acids; liver regeneration; mice
( diet; phospholipids; fatty acids; liver regeneration; mice )
Sažetak:
Two groups of C57Bl mice, aged 8-10 weeks (5-8 animals per group) were fed, over 21 days, diets enriched with olive oil (5% addition to standard pellet, w/w), containing predominantly oleic acid (C18:1n-9), or standard pellet diet. The animals were one-third partially hepatectomised (1/3 pHx) under aether anaesthesia, sacrified by cervical disslocation 6th, 12th, 24th, and 48th hours later, when the brains were carefully removed. Total lipids were extracted from tissue samples with a chloroform/methanol (2:1, v/v) mixture according to Folch et al. Phospholipids (PL) were separated and purified from brain's total lipid mixture using solid phase extraction (SPE) procedure on aminopropylsilica columns. Fatty acids (FAs) of polar lipids were analysed in the form of fatty acid methyl esters (FAMEs) by gas chromatography (GC). Significant increases in the amounts of arachidonic and docosahexaenoic acids were detected in olive oil fed groups, 12 and 48 hours after pHx.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 062-0621341-0061
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Kemija,Temeljne medicinske znanosti
Upisao u CROSBI: prado@medri.hr (prado@medri.hr), 23. Vel. 2011. u 14:27 sati



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