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Pregled bibliografske jedinice broj: 508038

Časopis

Autori: Marković, Ivan; Marković-Devčić, Branka; Pavlović, Nediljko
Naslov: The effect of synergism in decreased hydrolysis of biopolymers with enzyme overdosage
( The effect of synergism in decreased hydrolysis of biopolymers with enzyme overdosage )
Izvornik: Process biochemistry (0032-9592) 38 (2002), 5; 783-790
Vrsta rada: članak
Ključne riječi: Enzyme overdosage; Synergism; alfa-Amylase; Starch; Hydrolisis; Biopolymer
( Enzyme overdosage; Synergism; alfa-Amylase; Starch; Hydrolisis; Biopolymer )
Sažetak:
Enzyme overdosage in biopolymer or complex (natural) substrates hydrolysis may decrease product yield, degree of conversion, filtration rate and prolong saccharification. Generally, enzyme efficiency may also be reduced. Synergistic action in multi-enzyme systems depends on the enzyme ratio. Using a model system of starch and fungal (Aspergillus oryzae) and bacterial (Bacillus subtilis ) a-amylases, it was observed that the highest degree of synergistic effect (DSE) of 2.10 was achieved with the combination of 0.2 mU/ ml fungal and 0.8 mU/ml bacterial a-amylase. This enzyme ratio was used as the initial value in the studies of enzyme overdosage. However, when the concentration of the bacterial enzyme was increased and the concentration of the fungal enzyme remained constant, the synergistic action of the enzymes decreased. As a consequence, reducing sugar released during hydrolysis goes through a maximum. The decrease in hydrolysis with enzyme overdosage depended not only on the enzyme ratio alteration, but also on the reaction time. The consequences of a decreased biopolymers degradation with enzyme overdosage in industrial processes and in animal nutrition are considered. The effect of decreased synergism in decreased hydrolysis efficiency with enzyme overdosage in relation to enzyme production and use are discussed.
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
SCI-EXP, SSCI i/ili A&HCI
Kategorija: Znanstveni
Znanstvena područja:
Kemijsko inženjerstvo,Biotehnologija,Prehrambena tehnologija
URL Internet adrese: http://dx.doi.org/10.1016/S0032-9592(02)00223-6
http://http://www.sciencedirect.com/science/article/pii/S0032959202002236
http://http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271450&_user=3875467&_pii=S0032959202002236&_check=y&_origin=article&_zone=toolbar&_coverDate=31-Dec-2002&view=c&originContentFamily=serial&wchp=dGLbVlB-zSkzk&md5=cfe30c4575c3ede3f7e0c8be74aad811/1-s2.0-S0032959202002236-main.pdf
Broj citata:
Altmetric:
DOI: 10.1016/S0032-9592(02)00223-6
URL cjelovitog teksta: http://dx.doi.org/10.1016/S0032-9592(02)00223-6
Google Scholar: The effect of synergism in decreased hydrolysis of biopolymers with enzyme overdosage
Upisao u CROSBI: npavlovic@imz.hr (npavlovic@imz.hr), 29. Ožu. 2011. u 13:27 sati



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