crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 519317

Zbornik radova

Autori: Musani, Vesna; Sabol, Maja; Ozretić, Petar; Car, Diana; Leović, Dinko; Levacic Cvok, Mirela; Levanat, Sonja
Naslov: Simultaneous loss of heterozygosity and point mutation detection with high resolution melting
( Simultaneous loss of heterozygosity and point mutation detection with high resolution melting )
Izvornik: Eur J Hum Genet / van Ommen, G-J B (ur.). - Nature Publishing Group , 2011. 438-438.
ISSN: 1018-4813
Skup: European Human Genetics Conference 2011
Mjesto i datum: Amsterdam, Nizozemska, 28-31.05.2011
Ključne riječi: PTCH1; HRM; LOH
( PTCH1; HRM; LOH )
Sažetak:
Basal-cell carcinoma (BCC) is the most common type of skin cancer, and shows a continuously increasing incidence. BCCs occur predominantly on head and neck of elderly fair-skinned people, with increased occurrence on the trunk (torso) in recent years. Patched (PTCH1) gene is a tumor suppressor gene most often associated with development of BCCs. It is a member of the Hh-Gli signaling pathway, a pathway that plays a major role in embryonic development and stem cell maintenance. Loss of heterozygosity (LOH) of tumor suppressor genes is a frequent event in tumorigenesis, and is most often analyzed by microsatellite typing. Here we offer a fast and efficient method for simultaneous SNP genotyping and mutation scanning, which can also be used for LOH detection. High resolution melting (HRM) analysis is based on differences in melting curves caused by variations in nucleotide sequence during the heating (melting) of the PCR product in the presence of the intercalating dye. Based on our previous experience with HRM, we expanded our mutation screening to LOH analysis with a study of 25 BCC samples. We also compared the LOH results obtained with HRM with those of the conventional microsatellite typing for several STR markers in PTCH1 region. HRM is an excellent method for both point mutation detection and LOH analysis, with point mutations found in 40%, and LOH in 50% of samples, and the interrater agreement between microsatellite and HRM LOH analyses of κ value of 0.869, what can be interpreted as very good agreement between the methods.
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Nema recenziju
Projekt / tema: 098-0982464-2461, 108-1080057-0043
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
Upisao u CROSBI: vmusani@irb.hr (vmusani@irb.hr), 11. Srp. 2011. u 12:10 sati



Verzija za printanje   za tiskati


upomoc
foot_4