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Pregled bibliografske jedinice broj: 522999

Časopis

Autori: Špoljarić, Dubravka; Čipak, Ana; Horvatić, Janja; Andrišić, Luka; Waeg, Georg; Žarković, Neven; Jaganjac, Morana
Naslov: Endogenous 4-hydroxy-2-nonenal in microalga Chlorella kessleri acts as a bioactive indicator of pollution with common herbicides and growth regulating factor of hormesis
Izvornik: Aquatic toxicology (0166-445X) 105 (2011), 3/4; 552-558
Vrsta rada: članak
Ključne riječi: Chlorella kessleri; S-metolachlor; terbuthylazine; reactive oxygen species; 4-hydroxy-2-nonenal; hormesis
Sažetak:
Oxidative stress, i.e. excessive production of reactive oxygen species (ROS), leads to lipid peroxidation and to formation of reactive aldehydes (e.g. 4-hydroxy-2-nonenal ; HNE), which act as second messengers of free radicals. It was previously shown that herbicides can induce ROS production in algal cells. In the current paper, the unicellular green microalga Chlorella kessleri was used to study the effect of two herbicides (S-metolachlor and terbuthylazine) and hydrogen peroxide (H2O2) on oxidative stress induction, HNE formation, chlorophyll content and the cell growth. Production of HNE was detected in this study for the first time in the cells of unicellular green algae using the antibody specific for the HNE-histidine adducts revealing the HNE-histidine adducts even in untreated, control C. kessleri. Exposure of algal cells to herbicides and H2O2 increased the ROS production, modifying production of HNE. Namely, 4 h upon treatment the levels of HNE-histidine conjugates were below controls. However, their amount increased afterwards. The increase of HNE levels in algae was followed by their increased growth rate, as was previously described for human carcinoma cells. Hence, changes in the cellular HNE content upon herbicide treatment inducing lipid oxidative stress and alterations in cellular growth rate of C. kessleri resemble adaptation of malignant cells to the HNE treatment. Therefore, as an addition to the standard toxicity tests, the evaluation of HNE-protein adducts in C. kessleri might indicate environmental pollution with lipid peroxidation-inducing herbicides. Finally, C. kessleri might be a convenient experimental model to further study cellular hormetic adaptation to oxidative stress-derived aldehydes.
Projekt / tema: 098-0982464-2519, 285-0000000-3175
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Biologija
URL Internet adrese: http://dx.doi.org/10.1016/j.aquatox.2011.08.007
http://www.sciencedirect.com/science/article/pii/S0166445X11002293
http://ac.els-cdn.com/S0166445X11002293/1-s2.0-S0166445X11002293-main.pdf?_tid=0088bec8447d5700fed36beb394373ed&acdnat=1332335625_8a08c1e17f11d232a49b6d52526e6236
Broj citata:
Altmetric:
DOI: 10.1016/j.aquatox.2011.08.007
URL cjelovitog teksta: http://dx.doi.org/10.1016/j.aquatox.2011.08.007
Google Scholar: Endogenous 4-hydroxy-2-nonenal in microalga Chlorella kessleri acts as a bioactive indicator of pollution with common herbicides and growth regulating factor of hormesis
Upisao u CROSBI: dspoljaric@unios.hr (dspoljaric@unios.hr), 24. Kol. 2011. u 09:11 sati



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