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Pregled bibliografske jedinice broj: 536545

Časopis

Autori: Grubišić, Leon; Šegvić-Bubić, Tanja; Lepen Pleić, Ivana; Mišlov, Krstina; Tičina, Vjekoslav; Katavić, Ivan; Mladineo, Ivona
Naslov: Morphological and genetic identification of spontaneously spawned larvae of captive Bluefin Tuna Thunnus thynnus in the Adriatic Sea
( Morphological and genetic identification of spontaneously spawned larvae of captive Bluefin Tuna Thunnus thynnus in the Adriatic Sea )
Izvornik: Fisheries (0363-2415) 38 (2013), 9; 410-417
Vrsta rada: članak
Ključne riječi: Bluefin Tuna; Thunnus thynnus; spawning; Adriatic Sea
( Bluefin Tuna; Thunnus thynnus; spawning; Adriatic Sea )
Sažetak:
The spawning behavior of captive Bluefin Tuna (Thunnus thynnus) was observed in cages at an Adriatic facility in summer 2011. Approximately 20, 000 eggs were collected from cages and transferred to a land-based nursery, with an estimated fertilization success rate of 80%. Eggs were spherical with a diameter of 1, 035.06 ± 20.16 μm and were pelagic with a single oil globule (227.63 ± 8.07 μm). Newly hatched larvae (3.08 ± 0.14 mm total length) followed scombrid larval development: melanophores were scattered over the body, head, notochord, and yolk, except finfold ; eye pigmentation was observed 1.5 days posthatch, pectoral fins started to appear, and two-thirds of the yolk sac were absorbed ; the mouth developed at 2.5 days posthatch, and larvae began feeding upon enriched rotifers. After mortalities occurred in the nursery (5 days posthatch), DNA was extracted from a sample of larvae. An 890 base pair fragment of the mitochondrial partial control region was amplified and sequenced to genetically confirm fish species identity. Based on multiple sequence alignment, larval sequences showed no ambiguities to the T. thynnus mitochondrial control region, thereby confirming spontaneous spawning in captivity. Although such an event has previously been reported in the Mediterranean, this is the first report supported by both morphological and genetic data.
Projekt / tema: 001-0000000-3633, 001-0010501-0560
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Veterinarska medicina,Biotehnologija
URL Internet adrese: http://www.tandfonline.com/doi/full/10.1080/03632415.2013.826201#.U1pIWqJqOoY
Broj citata:
Altmetric:
DOI: 10.1080/03632415.2013.826201
URL cjelovitog teksta:
Google Scholar: Morphological and genetic identification of spontaneously spawned larvae of captive Bluefin Tuna Thunnus thynnus in the Adriatic Sea
Upisao u CROSBI: mladineo@izor.hr (mladineo@izor.hr), 10. Stu. 2011. u 14:28 sati



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