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Pregled bibliografske jedinice broj: 540638

Zbornik radova

Autori: Tadin, Ante; Matijević, Ivana; Antonović, Ivan; Pintera, Alojzija; Jug, Renata; Markotić, Alemka
Naslov: Chemokines and metalloproteinases gene expression in early infection of MRC-5 cells with Mycoplasma pneumoniae
( Chemokines and metalloproteinases gene expression in early infection of MRC-5 cells with Mycoplasma pneumoniae )
Izvornik: Book of Abstracts - 2009 Annual Meeting of the Croatian Immunological Society
Skup: 2009 Annual Meeting of the Croatian Immunological Society
Mjesto i datum: Starigrad Paklenica, Hrvatska, 1.- 4. listopad 2009.
Ključne riječi: Chemokines; metalloproteinases; MRC-5 cells; Mycoplasma pneumoniae
( Chemokines; metalloproteinases; MRC-5 cells; Mycoplasma pneumoniae )
Sažetak:
Bacteria Mycoplasma pneumoniae causes tracheobronchitis and atypical pneumonia in humans. Important component of M. pneumoniae immunopatogenesis is induction of proinflammatory chemokines secreted particularly by lung macrophages and endothelial cells. However, no information is available about the role of lung fibroblasts in infection with M. pneumoniae. In our study we investigated the ability of M. pneumoniae to induce gene expression of chemokines IL-8, MCP-1, MIP-1β and metalloproteinases 2 and 9 (MMP-2 and MMP-9) in human lung fibroblast like MRC-5 cells in early phase of infection. Additionally, we looked for immunomodulatory effects of antibiotics which are commonly used in the treatment of atypical pneumonia caused by M. pneumoniae. For that purpose, MRC-5 cells were infected with M. pneumoniae and also treated with antibiotics azithromycin, ciprofloxacin and doxycycline. Two time points, two and six hours after infection were checked. After isolation of mRNA and synthesis of cDNA, detection of sequences specific for chemokines and MMPs was performed by real time PCR (Roche Applied Science LightCycler System). The most striking effect of M. pneumoniae on MRC-5 cells was induction of IL-8 expression. MCP-1 and MMP-2 genes did not show significantly higher expression after infection with M. pneumoniae and MRC-5 cells showed not to express MIP-1β and MMP-9 genes. Azithromycin, ciprofloxacin and doxycycline showed to have immunomodulatory activity. Azythromycin enhanced expression of MCP-1 and all three antibiotics enhanced expression of IL-8 in the initial phase of infection.
Vrsta sudjelovanja: Predavanje
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Domaća recenzija
Projekt / tema: 143-1430115-0103
Izvorni jezik: eng
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti,Kliničke medicinske znanosti
Upisao u CROSBI: apavelic@bfm.hr (apavelic@bfm.hr), 15. Stu. 2011. u 15:12 sati



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