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Pregled bibliografske jedinice broj: 548164

Časopis

Autori: Čipak, Ana; Borović, Suzana; Jaganjac, Morana; Bresgen, Nikolaus; Kirac, Iva; Grbeša, Ivana; Mrakovčić, Lidija; Cindrić, Marina; Šćukanec-Špoljar, Mira; Gall-Trošelj, Koraljka; Ćorić, Marijana; Eckl, Peter; Žarković, Neven
Naslov: Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells
Izvornik: Acta biochimica Polonica (0001-527X) 57 (2010), 2; 185-191
Vrsta rada: članak
Ključne riječi: 4-hydroxynonenal; spleen; hepatocyte; novel-liver derived cell line
Sažetak:
Liver is a unique mammalian organ with a great capacity of regeneration related to its function. After surgical resection or injury, hepatic cells, especially hepatocytes, can proliferate rapidly to repair the damage and to regenerate the structure without affecting the function of the liver. Loss of catalase activity during regeneration indicates that oxidative stress is present in the liver not only in pathological conditions but also as a “physiological” factor during regeneration. As we have shown in our previous work, liver stem cell-like cells treated with 4-hydroxynonenal (HNE), a cytotoxic and growth regulating lipid peroxidation product, recover in the presence of spleen cells. In the current study we characterized this novel cell line as liver-derived progenitor/oval-like cells, (LDP/OCs), i.e. functional liver stem-like cells. We showed that LDP/OC were OV6 positive, with abundant glycogen content in the cytoplasm and expressed α-fetoprotein, albumin, biliverdin reductase and γ-glutamyl transferase. Also, we compared their growth in vitro with the growth of cultured primary hepatocytes stressed with HNE and co-cultured with autologous spleen cells. The influence of spleen cells on HNE-treated primary hepatocytes and on LDP/OCs showed that spleen cells support in a similar manner the recovery of both types of liver cells indicating their important role in regeneration. Hence, LDP/OC cells may provide a valuable tool to study cell interactions and the role on HNE in liver regeneration.
Projekt / tema: 108-0532264-0048, 098-0982464-2511, 108-0000000-0028, 098-0982464-2519
Izvorni jezik: ENG
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bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
URL Internet adrese: http://www.actabp.pl/pdf/2_2010/185.pdf
URL cjelovitog teksta:
Google Scholar: Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells
Upisao u CROSBI: lbrcic@mef.hr (lbrcic@mef.hr), 21. Stu. 2011. u 13:55 sati



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