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Pregled bibliografske jedinice broj: 561369

Časopis

Autori: Jani, A.; ...; Pačić, Arijana; Ljubanović, Danica; Edelstein C.L.
Naslov: Perfusion storage reduces apoptosis in a porcine kidney model of donation after cardiac death.
( Perfusion storage reduces apoptosis in a porcine kidney model of donation after cardiac death. )
Izvornik: Transplantation (0041-1337) 91 (2011), 2; 169-175
Vrsta rada: članak
Ključne riječi: cardiac death; kidney transplantation; cold ischemia; delayed graft function
( cardiac death; kidney transplantation; cold ischemia; delayed graft function )
Sažetak:
Donation after cardiac death (DCD) kidneys suffer a high incidence of delayed graft function attributable to warm ischemia and cold ischemia (CI). Neither the mechanism of injury nor type of cell death has been described. Clinical studies suggest that perfusion storage (PS) of DCD kidneys may reduce injury although the mechanism of protection is unknown. In a porcine model of DCD, we hypothesized that DCD kidneys have increased caspase-1 due to warm ischemia (WI) and increased caspase-3 and apoptosis due to CI. Male Yorkshire pigs subjected to cardiac death were perfused with cold University of Wisconsin solution. The perfused kidneys were removed and stored in cold University of Wisconsin solution for 24 hr. Kidney biopsies were obtained before cardiac death and at 0 and 24 hr of CI. There was an increase in caspase-1 activity due to WI before cold preservation. CI was associated with a massive increase in apoptosis, caspase-3/7 activity, and caspase-3 protein expression. Next, we hypothesized that PS would protect against apoptosis. We compared DCD kidneys subjected to static versus PS for 24 hr. PS significantly reduced proximal tubular apoptosis and was associated with increased B-cell lymphoma-extra large, and hypoxia-inducible transcription factor-1α. These findings suggest that in DCD kidneys, WI preferentially activates caspase-1, whereas CI activates caspase-3 and causes apoptosis. PS may protect DCD kidneys through activation of antiapoptotic pathways involving B-cell lymphoma-extra large and hypoxia-inducible transcription factor-1α.
Projekt / tema: 198-0000000-3355
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
URL Internet adrese: http://journals.lww.com/transplantjournal/Abstract/2011/01270/Perfusion_Storage_Reduces_Apoptosis_in_a_Porcine.7.aspx
Broj citata:
Altmetric:
DOI: 10.1097/TP.0b013e3182013753
URL cjelovitog teksta:
Google Scholar: Perfusion storage reduces apoptosis in a porcine kidney model of donation after cardiac death.
Upisao u CROSBI: dljubanovic@mef.hr (dljubanovic@mef.hr), 29. Stu. 2011. u 02:56 sati



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