Oxidation of N-Cbz-3-aminopropanol catalyzed by yeast alcohol dehydrogenase (YADH) was carried out in this work. Since ADH requires coenzyme for its activity a coenzyme regeneration system was used to reduce the coenzyme consumption. Addition of co-substrate acetone was used for that purpose (Scheme 1). Scheme 1. The reaction scheme. YADH used in this work was isolated from baker's yeast. Due to ADH instability at higher pH values, pH 8.6 in glycine NaOH buffer was used for all measurements. The reaction kinetics was determined by using the initial reaction rate method. From experimental data kinetic parameters were estimated (Vm, Km, Ki) by using the non-linear regression analysis. Mathematical model of the process, which consisted of the kinetic model and mass balances in the batch reactor, was developed. Oxidation was carried out in the batch reactor without and with coenzyme regeneration. The conversion of substrate with coenzyme regeneration was around 60%. The mathematical model described the data well.

Biotehnologija