engleski

MMP-9 CONCENTRATION AND MMP-9 GENE POLYMORPHISMS IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

Matrix metalloproteinase-9 (MMP-9) is a zinc-dependent endopeptidase capable of cleaving several constituents of extracellular matrix, such as collagen, gelatin, elastin, etc. Therefore, MPP-9 plays an important physiological role in lung extracellular matrix remodeling and repair. In the lungs of patients with chronic obstructive pulmonary disease (COPD) increased degradation of extracellular matrix and airway remodeling are present. Several studies showed increased levels of MMP-9 in alveolar macrophages, neutrophils and sputum of COPD patients. In this study, we measured MMP-9 concentration in plasma samples of COPD patients using a commercially available ELISA kit. We found statistically significant increase in the median value of MMP-9 concentration in COPD patients (204.1 (115.7-351.2) ng/mL ; N=59) comparing to healthy controls (70.2 (52.4-104.9) ng/mL ; N=21), P<0.001. Previous studies suggested that presence of genetic variations in MMP-9 gene might be associated with changes in MMP-9 activity. However, no consistent results were found in COPD patients. Hence, we analyzed 2 polymorphisms in promotor region (-1562 C/T polymorphism and number of CA repeats) and a +2679 A/G (Gln279Arg) polymorphism in exon 6 of MMP-9 gene. The results show no statistically significant differences in distribution of genotype or allele frequencies for -1562 C/T (P=0.199 and P=1.000, respectively) and +2679 A/G (P=0.223 and P=1.000, respectively) polymorphisms between patients with COPD (N=137) and control subjects (N=43). Likewise, there were no statistically significant differences in either genotypes or number of CA repeats (P=0.199 and P=1.000, respectively). These results indicate no association of selected genetic variations in promotor region and exon 6 of MMP-9 gene with increased MMP-9 concentration in plasma of COPD patients.

matrix metalloproteinase-9 ; chronic obstructive pulmonary disease ; polymorphisms

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