crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 593447

Zbornik radova

Autori: Gršković Branka; Mršić Gordan; Zidkova Anastassiya; Vrdoljak Andro; Stenzl Vlastimil; Popović Maja; Primorac Dragan
Naslov: Central Croatian population data of eight X-linked markers in four linkage groups
Izvornik: DNA in Forensics 2012 "Exploring the Phylogenies" Abstract book and Conference program / Walther Parson and Lutz Roewer (ur.). - Innsbruck :
Skup: DNA in Forensics 2012 "Exploring the Phylogenies" 5th International EMPOP Meeting and 8th International Forensic Y‐User Workshop
Mjesto i datum: Innsbruck, Austrija, 06-08.09.2012.
Ključne riječi: Chromosome X; Short Tandem Repeat; Polymorphism; Population data; Kinship Testing; Central Croatia
Sažetak:
The analysis of short tandem repeats (STRs) located on X chromosome shown to be particularly powerful in solving complex kinship cases. The aim of this study was to analyze 8 X-STRs in central Croatian population. We carried out a statistical analysis of the data from previously performed genetic analyses collected during routine forensic work by the Forensic Science Centre ‘‘Ivan Vučetić’’. A total of 99 unrelated healthy women and 78 men from central Croatia were typed using Mentype Argus X-8 PCR amplification kit. The allele and haplotype frequencies were determined by counting. Haplotype frequencies were calculated only in male samples. Arlequin 3.5 software was used to assess Hardy-Weinberg equilibrium (HWE), linkage disequilibrium (LD), observed and expected heterozygosity. For HWE and LD tests Bonferroni correction was used. Power of discrimination (PD) for males and females was calculated according to Desmarais. Polymorphism information content (PIC) was determined using online database ChrX-STR.org that calculates population-genetic data. In female samples deviations from HWE (p>0.00625) for each locus were not found. LD test performed on female and male samples, revealed no significant association between markers (p>0.00178). In 78 men, 37, 30, 35 and 30 haplotypes were found for linkage groups 1-4. Locus DXS10135 was the most polymorphic (PIC=0.9306). DXS7423 and DXS8378 loci showed the lowest values (0.6316 and 0.6447). PIC for whole marker set was 0.999998. PD varied from 0.6922 to 0.9345 in male and from 0.8447 to 0.9918 in female samples. Combined PD reached 99.9999% in males and 99.99999999% in females. Further analyses that will include more X-STR loci are needed to increase discrimination power for kinship and paternity testing as well as population genetics studies. Nevertheless, Mentype Argus X-8 kit can be used as an additional marker panel for forensic identification and complex kinship cases in central Croatian population.
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Izvorni jezik: ENG
Kategorija: Ostalo
Znanstvena područja:
Biologija,Temeljne medicinske znanosti
Upisao u CROSBI: gmrsic@public.hr (gmrsic@public.hr), 17. Ruj. 2012. u 15:32 sati



Verzija za printanje   za tiskati


upomoc
foot_4