crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 596558

Časopis

Autori: Vásquez-Garzón, Verónica R.; Rouimi, Patrick; Jouanin, Isabelle; Waeg, Georg; Žarković, Neven; Villa-Treviño, Saul; Guéraud, Françoise
Naslov: Evaluation of three simple direct or indirect carbonyl detection methods for characterization of oxidative modifications of proteins
Izvornik: Toxicology mechanisms and methods (1537-6516) 22 (2012), 4; 296-304
Vrsta rada: članak
Ključne riječi: oxidative stress ; carbonylation ; hydroxynonenal
Sažetak:
Among disruptions induced by oxidative stress, modifications of proteins, particularly irreversible carbonylation, are associated with the development of several diseases, including cardiovascular diseases, neurodegenerative diseases, and cancer. Carbonylation of proteins can occur directly or indirectly through the adduction of lipid oxidation products. In this study, three classical and easy-to-perform techniques to detect direct or indirect carbonylation of proteins were compared. A model protein apomyoglobin and a complex mixture of rat liver cytosolic proteins were exposed to cumene hydroperoxide oxidation or adduction to the lipid peroxidation product 4-hydroxynonenal in order to test direct or indirect carbonylation, respectively. The technique using a specific anti-4-hydroxynonenal-histidine adduct antibody was effective to detect in vitro modification of model apomyoglobin and cytosolic proteins by 4-hydroxynonenal but not by direct carbonylation which was achieved by techniques using biotin-coupled hydrazide or dinitrophenylhydrazine derivatization of carbonyls. Sequential use of these methods enabled the detection of both direct and indirect carbonyl modification in proteins, although constitutively biotinylated proteins were detected by biotin-hydrazide. Although rather classical and efficient, methods for carbonyl detection on proteins in oxidative stress studies may be biased by some artifactual detections and complicated by proteins multimerizations. The use of more and more specific available antibodies is recommended to complete detection of lipid peroxidation product adducts on proteins.
Projekt / tema: 098-0982464-2519
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Kemija
URL Internet adrese: http://www.tandfonline.com/doi/full/10.3109/15376516.2012.657258
Broj citata:
Altmetric:
DOI: 10.3109/15376516.2012.657258
URL cjelovitog teksta:
Google Scholar: Evaluation of three simple direct or indirect carbonyl detection methods for characterization of oxidative modifications of proteins
Upisao u CROSBI: mstroser@mef.hr (mstroser@mef.hr), 4. Lis. 2012. u 08:33 sati



  Verzija za printanje   za tiskati


upomoc
foot_4