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Pregled bibliografske jedinice broj: 603531

Časopis

Autori: Ratkaj, Ivana; Bujak, Maro; Jurišić, Davor; Baus Lončar, Mirela; Bendelja, Krešo; Pavelić, Krešimir; Kraljević Pavelić, Sandra
Naslov: Microarray Analysis of Dupuytren’s Disease Cells: The Profibrogenic Role of the TGF-β Inducible p38 MAPK Pathway
Izvornik: Cellular physiology and biochemistry (1015-8987) 30 (2012), 4; 927-942
Vrsta rada: članak
Ključne riječi: Dupuytren’s Disease; Myofibroblasts; Differentiation; p38; Akt; CD90
Sažetak:
Dupuytren’s disease (DD) is a nodular palmar fibromatosis that causes irreversible permanent contracture of fingers and results in the loss of hand function. Surgery still remains the only available solution for DD patients but cannot permanently cure the disease nor reduce high recurrence rates. With this rationale, we designed a study aimed at an improved understanding of the molecular mechanisms underlying DD. Our major focus was an analysis of the global gene expression profile and signalling pathways in DD cells with the aim of identifying novel biomarkers and/or therapeutic targets. Primary cells were cultured from surgically removed diseased and healthy tissue. Microarray expression analysis (HG-U133A array, Affymetrix) and qPCR was performed with total RNA isolated from primary DD cells. Mechanistic studies involving inhibition of p38 phosphorylation were performed on normal human fibroblasts’ and primary DD cells’ in vitro models. Expression of stem cell markers in primary fibroblasts/myofibroblasts was assessed as well. We identified 3 p38MAPK signalling pathway regulatory genes, THBS1, GADD45α and NUAK1, all involved in cellular proliferation and production of the extracellular matrix proteins. Inhibition of the p38MAPK signalling pathway induced down-regulation of myofibroblast markers, α-smooth muscle actin and palladin. A stem-cell like subpopulation positive for CD90 marker was identified among primary DD cells. The study reveals involvement of the p38 MAPK pathway as a possible signalling cascade in the pathogenesis of Dupuytren’s disease. Moreover, a particular stem cell-like CD90+ subpopulation was identified that might contribute to DD development.
Projekt / tema: 14V09809, 335-0000000-3532, 335-0982464-2393
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Temeljne medicinske znanosti
Puni text rada: 603531.Ratkaj_Dupuytren_2012.pdf (tekst priložen 15. Stu. 2012. u 17:39 sati)
URL Internet adrese: http://content.karger.com/ProdukteDB/produkte.asp?doi=10.1159/000341470
Broj citata:
Altmetric:
DOI: 10.1159/000341470
URL cjelovitog teksta:
Google Scholar: Microarray Analysis of Dupuytren’s Disease Cells: The Profibrogenic Role of the TGF-β Inducible p38 MAPK Pathway
Upisao u CROSBI: iratkaj@uniri.hr (iratkaj@uniri.hr), 15. Stu. 2012. u 17:39 sati



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