crta
Hrvatska znanstvena Sekcija img
bibliografija
3 gif
 Naslovna
 O projektu
 FAQ
 Kontakt
4 gif
Pregledavanje radova
Jednostavno pretraživanje
Napredno pretraživanje
Skupni podaci
Upis novih radova
Upute
Ispravci prijavljenih radova
Ostale bibliografije
Slični projekti
 Bibliografske baze podataka

Pregled bibliografske jedinice broj: 625156

Časopis

Autori: Zaja, Roko; Terzić, Senka; Senta, Ivan; Lončar, Jovica; Popović, Marta; Ahel, Marijan; Smital, Tvrtko
Naslov: Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments
( Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments )
Izvornik: Environmental science & technology (0013-936X) 47 (2013), 9; 4813-4821
Vrsta rada: članak
Ključne riječi: multixenobiotic resistance ; P-glycoprotein inhibitors ; contaminated sediments ; effect-directed analysis (EDA) ; nonionic surfactants
( multixenobiotic resistance ; P-glycoprotein inhibitors ; contaminated sediments ; effect-directed analysis (EDA) ; nonionic surfactants )
Sažetak:
P-glycoprotein (P-gp, ABCB1) is an important part of the multixenobiotic resistance (MXR) defense system in aquatic organisms. The main goal of this study was identification of P-gp inhibitors in contaminated sediments using the effect-directed analysis (EDA) approach. The samples were collected from the Gorjak creek (Zagreb, Croatia), a recipient of wastewater effluents from the pharmaceutical industry. Sediment samples were extracted and fractionated using two-tiered approach. Resulting non-polar, medium polar and polar fractions were tested on the inhibition of P-gp activity using P-gp overexpressing PLHC-1/dox cells and calcein-AM as model substrate. The obtained EC50 values - up to 757 μg/g, expressed in toxicity equivalents of model P-gp inhibitor cyclosporine A - revealed high inhibitory potential of polar fractions of investigated sediments and clearly reflected the impact of pharmaceutical wastewater. P-gp specific ATPase assay and the cytotoxicity modulation experiments with colchicine indicated that most of the observed P-gp inhibition was due to the presence of non-competitive inhibitors. A detailed chemical analysis by ultrahigh-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QToFMS) revealed nonionic surfactants, including alcohol polyethoxylates (LAEOs) and polypropylene glycols (PPGs), as the major components of the most active sub-fractions. Testing of several LAEO and PPG commercial mixtures confirmed their potential to inhibit the fish P-glycoprotein and modulate toxicity of other xenobiotics present in complex environmental samples.
Projekt / tema: 098-0982934-2712, 098-0982934-2745
Izvorni jezik: eng
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Geologija,Biologija
URL Internet adrese: http://pubs.acs.org/doi/abs/10.1021/es400334t
Broj citata:
Altmetric:
DOI: 10.1021/es400334t
URL cjelovitog teksta:
Google Scholar: Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments
Upisao u CROSBI: Tvrtko Smital (Tvrtko.Smital@irb.hr), 10. Tra. 2013. u 11:39 sati



  Verzija za printanje   za tiskati


upomoc
foot_4