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Pregled bibliografske jedinice broj: 632858

Časopis

Autori: Gršković, Branka; Zrnec, Dario; Popović, Maja; Petek, Maja Jelena; Primorac, Dragan; Mršić, Gordan
Naslov: Effect of ultraviolet C radiation on biological samples
Izvornik: Croatian medical journal (0353-9504) 54 (2013), 3; 263-271
Vrsta rada: članak
Ključne riječi: ultraviolet C radiation; biological samples; blood; saliva; semen; decontamination; forensic science
Sažetak:
Aim was to examine the influence of ultraviolet C (UVC) radiation on blood, saliva, semen, and naked DNA samples for preventing DNA cross-contamination on working surfaces in laboratories. Blood, saliva, semen, and DNA isolated from buccal swab samples were obtained from a single male donor and applied to the laboratory working surfaces. UVC radiation was applied to these diluted and undiluted samples with or without previous decontamination of the working surfaces with 10% sodium hypochlorite and 20% ethanol. Genomic DNA was extracted using Chelex. After quantification, DNA was amplified using the AmpFlSTR® NGM PCR Amplification Kit. We tested and statistically analyzed DNA concentration, UVC dose, sample volume, radiation time, the number of correctly detected alleles on genetic loci, and the number of correctly detected alleles in four groups in which 16 loci were divided. When working surfaces were not decontaminated and were treated only with UVC radiation in the laboratory, the genetic profile for naked DNA could not be obtained after 2 minutes of UVC radiation and for saliva after 42 hours. For blood and semen, a partial genetic profile was obtained even after 250 hours of UVC radiation in the laminar. When working surfaces were decontaminated with 10% sodium hypochlorite and 20% ethanol, genetic profile could not be obtained for naked DNA after 2 minutes, for saliva after 1 hour, for blood after 16 hours, and for semen after 8 hours of the UVC radiation in the laboratory. It is recommended to carefully and thoroughly clean working surfaces with 10% sodium hypochlorite and 20% ethanol followed by minimal 16-hour UVC exposure(dose approximately 4380 mJ/cm2) for complete and successful decontamination.
Projekt / tema: 053-0532265-2255
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
Current Contents Connect (CCC)
MEDLINE
Scopus
SCI-EXP, SSCI i/ili A&HCI
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Biologija,Temeljne medicinske znanosti,Veterinarska medicina
URL Internet adrese: http://www.cmj.hr/2013/54/3/23771757.htm
Broj citata:
Altmetric:
DOI: 10.3325/cmj.2013.54.263
URL cjelovitog teksta:
Google Scholar: Effect of ultraviolet C radiation on biological samples
Upisao u CROSBI: Gordan Mršić (Gordan.Mrsic@public.carnet.hr), 13. Lip. 2013. u 09:25 sati



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