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RELATION OF TOUCH DNA FROM DIFFERENT SURFACES WITH DONOR AGE AND GENDER

DNA can be transferred from palms to handled items. Different factors influence this process. The aim of this study was to investigate the influence of handling time, item texture, donor age and gender on quantity of DNA available from the touched items. The dependence of donor age and gender on touch DNA quantity deposits left by participants on items of different texture (paper, plastic, plastic coated metal) was also analyzed. 60 participants (30 men and 30 women) were included in this study. They were holding or rubbing items with their fingers, palm and a side of the palm of dominant hand in different time intervals. Items were swabbed with moistened cotton swab. Genomic DNA was isolated using Chelex. DNA concentration was determined by real-time polymerase chain reaction. The results showed that amount of participants’ DNA left on touched items was not correlated with the time interval. On contrary, it was dependent on item texture. The highest amount of touch DNA was transferred on plastic coated metal and the least on paper. In addition, participants between 35 and 44 years of age left the greatest amount of touch DNA, while participants between 25 and 34 years of age left the least. Moreover, man left higher DNA amount on handled items when compared to women. In conclusion, the property of leaving DNA on handled items is individual. Further research is needed to understand the process of DNA transfer from donors to handled items and factors that have an influence on it.

RELATION OF TOUCH DNA FROM DIFFERENT SURFACES WITH DONOR AGE AND GENDER

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