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Autori: Ilić, Nada; Novković, Mario; Guida, Filomena; Xhindoli, Daniela; Benincasa, Monica; Tossi, Alessandro; Juretić, Davor
Naslov: Adepantins, computationally designed, glycine-rich peptide antibiotics, with low toxicity and very high G- selectivity
Izvornik: New Antimicrobials Workshop in Trieste, 25-26.5.2012 / Tossi, Alessandro (ur.). - Trieste : Edizioni Universita di Trieste , 2012. 37-38 (ISBN: 978-88-8303-379-7).
Skup: New Antimicrobials Project 2-nd Workshop. New Compounds & New Strategies for Antimicrobials
Mjesto i datum: Trst, Italija, 25-26.05.2012
Ključne riječi: adepantins; peptide antibiotics; therapeutic index
Sažetak:
Adepantins (ADPs) are glycine-rich antimicrobial peptides (AMPs), constructed using the “Designer” algorithm, which is based on known characteristics of anuran AMPs [1]. Parameters used to predict the primary structure of ADPs include various physicochemical properties, such as lengthwise asymmetry and predicted therapeutic index (TI). ADP1 was previously tested and has shown high antimicrobial activity (MIC) against E. coli (2-4 microM) and low cytotoxic activity (HC50) against human red blood cells (480 microM). To confirm the high selectivity of peptides from this group, additional tests were performed for monomeric, dimeric and fluorescently labelled versions of ADP2 and ADP3, using different bacterial strains, host cells and model membrane systems. They are selective for Gram-negative bacterial cells (MIC = 0.5 - 4 microM) both with respect to human erythrocytes (HC50 > 400 microM for monomers), as with ADP1, and with respect to Gram-positive bacteria (MIC > 128 microM). Dimers have high haemolicity and exceptional antimicrobial activity. All adepantins structure as alpha helices when in contact with PG/dPG liposomes, while maintaining a random coil structure in the presence of PC/SM/Ch membranes. At sub-toxic concentrations, dimers exhibit much higher permeabilization of both bacterial membranes in various E. coli strains than monomers. The “Designer” algorithm thus identified novel AMPs less than 50% homologous to any other natural or synthetic AMP, highly selective for Gram-negative bacteria and with a high TI (up to 400). [1] D. Juretić, D. Vukičević, N. Ilić, N. Antcheva, A. Tossi, Computational design of highly selective antimicrobial peptides, J Chem Inf Model. 49, 2873-2882 (2009)
Vrsta sudjelovanja: Poster
Vrsta prezentacije u zborniku: Sažetak
Vrsta recenzije: Međunarodna recenzija
Projekt / tema: 177-1770495-0476
Izvorni jezik: ENG
Kategorija: Znanstveni
Znanstvena područja:
Fizika,Kemija,Biologija
Upisao u CROSBI: Davor Juretić (juretic@pmfst.hr), 13. Srp. 2013. u 13:31 sati



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