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Pregled bibliografske jedinice broj: 638745

Časopis

Autori: Janowska, Maria K.; Zubac, Ruben; Žagrović, Bojan
Naslov: Computational Analysis of Binding of the GBD Domain of WASP to Different Binding Partners
Izvornik: Croatica chemica acta (0011-1643) 84 (2011), 2; 211-220
Vrsta rada: članak
Ključne riječi: Wiskott-Aldrich syndrome protein; GBD domain; VCA domain; EspFU; molecular dynamics; GROMOS 45A3 force field
Sažetak:
The GTP-ase binding domain (GBD) of the signaling protein Wiskott-Aldrich Syndrome Protein (WASP) is intrinsically disordered and mutations in it have been linked with Wiskott-Aldrich Syndrome (WAS), an X-linked disorder characterized by thrombocytopenia, eczema and recurrent infections. Here, we use molecular dynamics simulations and the semi-empirical GROMOS 45A3 force field to study interaction of the GBD domain of WASP with a fragment of the protein EspFU as well as with the VCA domain of WASP (auto-inhibited state). EspFU is secreted and used by enterohaemorrhagic Escherichia coli to hijack eukaryotic cytoskeletal machinery, and it does so by competitively disrupting the auto-inhibitory interaction between GBD and VCA domains of WASP. In addition, naturally occurring mutations in the VCA domain cause different variants of WAS. Our simulations confirm that the EspFU domain binds the GBD domain similarly to the VCA domain, which explains why these two binding partners are competitive binders of the GBD domain. Furthermore, we propose a possible mechanism to explain the higher affinity of EspFU for the GBD domain. Finally, we show that the mutations in the VCA domain responsible for Wiskott-Aldrich syndrome can cause formation of β-sheets in the VCA domain. This effect, combined with the mutation-induced rearrangement of the salt bridge network, consequently disables tight binding between GBD and VCA domains. Overall, our results provide a microscopic, dynamic picture behind the two main ways through which the interactions involving the GBD domain of WASP participate in different disease processes. (doi: 10.5562/cca1806)
Projekt / tema: 177-1770495-0476
Izvorni jezik: ENG
Rad je indeksiran u
bazama podataka:
SCI-EXP, SSCI i/ili A&HCI
Current Contents Connect (CCC)
Scopus
Science Citation Index Expanded (SCI-EXP) (sastavni dio Web of Science Core Collectiona)
Kategorija: Znanstveni
Znanstvena područja:
Fizika,Kemija,Biologija
URL Internet adrese: http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=107219
Broj citata:
Altmetric:
DOI: 10.5562/cca1806
URL cjelovitog teksta:
Google Scholar: Computational Analysis of Binding of the GBD Domain of WASP to Different Binding Partners
Upisao u CROSBI: Davor Juretić (juretic@pmfst.hr), 31. Srp. 2013. u 12:50 sati



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