engleski

The effect of cyclosporine on number od CD25 plus inflammatory cells within colonic mucosa in a murin model of experimental colitis

Background: In intestinal inflammation, the inflammatory cells accounting within mucosa and submucosa express various functional surface molecules including the receptor for IL-2 (CD 25). The aim of this study was to investigate the effect of cyclosporine (CsA), given intreperitonealy i.p.) or in a form of enema (i.r.) on number of CD25 plus inflammatory cells within colonic mucosa and submucosa, in haptene- induced murine model of experimental colitis. Methods: Experimental colitis was induced in NMRI mice (Pliva Research Institute), using the enema of 0.2 percent solution od Dinitrofluorobenzen (DNFB) (Sigma, Saint Luois, USA) in 4:1 acetone to olive solution, and combined with previous skin sensitisation. Two experimental groups of animals (N equal 6-8) were treated with 3/mg/kg/ day) of CsA diluted with phosponate buffered saline (PBS), and given i.p. or i.r., 6 hours after induction of colitis and for consecutive 5 days. One control group was treated with PBS i.r., in the same manner, after induction of colitis. Second control group consisted of untreated healthy animals. On day 5, all animals were killed and entire colon of each animal was longitudinally dissected in two halves that were taken for histology (HE) and immunohystochemical analysis for CD25 (anti mouse IL-2R/CD25, Boehringer Biochemica Mannheim, Germany), respectively. Colonic lesions were assessed using histopathologic score ranging 0-30, and mean number of CD25 plus cells in 20 HPF (light microscopy) from each specimen was expressed as mean plus (min SD for each group of animals). Results: CsA was effective in diminishing the histologic extent of provoked colonic inflammation in experimental groups treated with dose of 3 mg/kg/day i.p. or i.r. (p less 0.05, Kruskal-Wallkis, ANOVA). There was no difference between experimental groups in regard to way of application of CsA. Induction of experimental colitis significantly increased the number of CD25 plus inflammatory cells, when compared to mucosa of healthy animals (p less 0.05, Mann- Whitney). CsA , applied i.p. or i.r. significantly decreased the number of CD25 plus inflammatory cells, when compared to control group with experimental colitis, treated with PBS i.r. (p less 0.05, Mann-Whitney). There was no difference between experimental groups in regard to way of application of CsA (i.p. or i.r.). Conclusion: CsA in dose 3 mg/kg/day is capable of diminishing the extent of haptene induced colonic inflammation when applied i.p. or i.r.. This effect is in part expressed in decreasing the number of CD25 plus cells within colonic mucosa and submucosa.

Cyclosporine; CD25 plus inflammatory cells; colonic mucosa; colitis

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